Anti-KITLG Polyclonal Antibody for WB, IF/ICC, ELISA - P21583

Core Product Specifications and Parameters

Biological Background: KITLG Function and Localization

• Kit ligand (KITLG), also known as stem cell factor (SCF), mast cell growth factor (MGF), and c-Kit ligand, is a cytokine that serves as the ligand for the receptor tyrosine kinase KIT.
• KITLG plays critical roles in cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis.
• Upon binding to KIT, KITLG activates multiple downstream signaling pathways, including PI3K-AKT, RAS-MAPK, JAK-STAT, and PLCγ pathways, coordinating a wide array of cellular responses.
• The protein is expressed as a membrane-bound form localized to the cell membrane, cytoplasm, cytoskeleton, and cell projections such as lamellipodia and filopodia, and can also be secreted as a soluble factor.
• KITLG is produced as a transmembrane protein that undergoes proteolytic cleavage to generate a soluble growth factor, with a molecular weight of approximately 31 kDa (UniProt: 30.9 kDa).
• It acts synergistically with other cytokines, particularly interleukins, to amplify signaling outputs in hematopoietic and immune cells.
• Clinically, mutations in KITLG are associated with disorders such as autosomal dominant nonsyndromic deafness (DFNA69) and Waardenburg syndrome type 2, highlighting its role in melanocyte and cochlear development.
• Keywords indicate its involvement in alternative splicing, cell adhesion, and disease variants, reflecting its multifunctional nature.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 120-190) falls within the extracellular domain of KITLG; therefore, the antibody is expected to recognize both the membrane-bound and soluble forms, assuming the epitope is accessible.
• For Western blotting, a band around 31 kDa is predicted; however, due to glycosylation and potential dimerization, observed molecular weight may vary; consider using reducing conditions and appropriate controls.
• For immunofluorescence/ICC, membrane and intracellular staining may be observed; permeabilization is required to detect cytoplasmic pools.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody recognizing a distinct epitope is recommended for sandwich ELISA development.
• Cross-reactivity with mouse and rat KITLG has been reported; however, validate in your specific sample system as sequence homology may affect affinity.
• Because KITLG is secreted, cell culture supernatants or serum samples may require optimization for detection.
• Use appropriate blocking agents to minimize non-specific binding, especially in tissues rich in mast cells.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a comprehensive sourcing solution for IVD raw materials, including validated polyclonal and monoclonal antibodies targeting critical biomarkers such as KITLG. Our one-stop platform supports your development pipeline with optimized antibody pairs, bulk ancillary reagents, and technical consulting—streamlining your path from discovery to diagnostic application.