Anti-KISS1R Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q969F8

Core Product Specifications and Parameters

Biological Background: KISS1R Function and Localization

• KISS1R (KiSS-1 receptor), also known as GPR54, AXOR12, and metastin receptor, is a G protein-coupled receptor that binds kisspeptins including kisspeptin-10 and metastin/kisspeptin-54 (PubMed:11457843, PubMed:11527393, PubMed:15020672). Related references: PMID:11457843, PMID:11527393, PMID:15020672

• The hypothalamic KISS1/KISS1R system is essential for puberty and reproduction by regulating gonadotropin-releasing hormone (GnRH) secretion from GnRH neurons (PubMed:12944565, PubMed:14573733, PubMed:15598687). Related references: PMID:12944565, PMID:14573733, PMID:15598687

• Upon kisspeptin binding, KISS1R activates G(q)-dependent phospholipase C (PLC) signaling, leading to intracellular calcium release and depolarization of GnRH neurons (PubMed:14573733, PubMed:15598687, PubMed:39151001). Related references: PMID:14573733, PMID:15598687, PMID:39151001

• KISS1R can also signal via β-arrestin, leading to MAPK/ERK cascade activation independently of G(q) (PubMed:25147978). Related references: PMID:25147978

• Kisspeptin-10-activated KISS1R recruits DUSP18 and SRC, resulting in DUSP18-mediated SRC dephosphorylation; in bone, this down-regulates osteoclast activity and suppresses bone resorption (PubMed:38346942, By similarity). Related references: PMID:38346942

• KISS1R is a multi-pass membrane glycoprotein localized to the cell membrane, and it is widely expressed in pancreas, placenta, spinal cord, various brain regions (including hypothalamus and pituitary), and at lower levels in kidney, lung, liver, and others.

• Mutations in KISS1R are linked to hypogonadotropic hypogonadism, underscoring its critical role in reproductive function.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 100-200 of human KISS1R (NP_115940.2). For Western blot (WB), use whole-cell lysates from tissues with positive expression, such as placenta or pancreas; expect a band around 43 kDa.
• In immunohistochemistry (IHC-P), antigen retrieval may be needed, and proper controls should be included. For immunofluorescence (IF/ICC), permeabilization protocols should be optimized.
• Since this is a rabbit polyclonal antibody, lot-to-lot variability may exist; validate the antibody in the intended application and species before large-scale experiments. Store at -20°C upon receipt (shipped on ice bags).

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides a streamlined pathway for diagnostic developers and research laboratories, offering comprehensive access to high-quality IVD raw materials, from validated antibody pairs to optimized polyclonal and monoclonal antibodies. For KISS1R-related reproductive biology and GPCR research, we can assist with sourcing bulk reagents, ancillary proteins, and custom rare-target antibodies. Partner with CamelBio to accelerate your assay development and bring reliable diagnostics from concept to clinic.