Anti-KISS1 (Kisspeptin-1) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15726

Core Product Specifications and Parameters

Biological Background: KISS1 Function and Localization

• KISS1 encodes the precursor protein kisspeptin-1, which is proteolytically processed into several biologically active peptides, including kisspeptin-54, kisspeptin-14, kisspeptin-13, and kisspeptin-10.
• Kisspeptins are endogenous ligands for the G protein-coupled receptor KISS1R/GPR54, activating G(q)-dependent phospholipase C signaling that increases intracellular calcium and depolarizes GnRH neurons. They also trigger G(q)-independent β-arrestin-mediated MAPK/ERK phosphorylation. Related references: PMID:11385580, PMID:11457843, PMID:11527393, PMID:15598687, PMID:22335740, PMID:25147978
• The hypothalamic KISS1/KISS1R signaling system is a central regulator of the reproductive axis, modulating gonadotropin-releasing hormone (GnRH) secretion and thereby controlling puberty and fertility. Related references: PMID:15219839, PMID:15598687, PMID:22335740
• Beyond reproduction, kisspeptins inhibit cell proliferation and migration, and play roles in trophoblast invasion during pregnancy and airway smooth muscle cell migration. Related references: PMID:11385580, PMID:11457843, PMID:11527393, PMID:15020672, PMID:38512807
• KISS1 is a secreted protein, consistent with its hormonal signaling function.
• KISS1 exhibits high expression in placenta, with lower levels in testis, pancreas, liver, and small intestine. Placental expression is upregulated in early gestation and molar pregnancies but downregulated in choriocarcinoma. Related references: PMID:8944003, PMID:11385580, PMID:11457843, PMID:12574233, PMID:15020672
• Post-translational modifications include signal peptide cleavage, amidation, and processing at dibasic sites to generate mature kisspeptins. Mutations in KISS1 are associated with hypogonadotropic hypogonadism.

Experimental Guidance and Technical Tips

• For Western blotting, a band near the predicted 15 kDa may be observed; however, potential glycosylation or precursor processing could produce additional bands. Validate using placenta or testis lysates as positive controls.
• In immunohistochemistry (IHC‑P), antigen retrieval may be required for this secreted target. Use placental tissue sections as a positive control and optimize blocking to reduce background.
• For ELISA applications, the antibody may be used for capture or detection; test peptide specificity and cross-reactivity with other kisspeptin isoforms using recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of high-quality IVD raw materials, serving diagnostic manufacturers and research laboratories with a comprehensive portfolio from concept to clinic. This anti-KISS1 polyclonal antibody supports research into reproductive endocrinology, cancer metastasis, and related assay development. Beyond individual antibodies, we offer validated antibody pairs, bulk ancillary reagents, and sourcing capabilities for rare targets—enabling streamlined procurement for your diagnostic and research workflows.