Anti-KIRREL Polyclonal Antibody for WB, ELISA - Q96J84

Core Product Specifications and Parameters

Biological Background: KIRREL1 Function and Localization

• KIRREL1 (Kin of IRRE-like protein 1), also known as NEPH1 or nephrin-like protein 1, is a transmembrane protein belonging to the immunoglobulin superfamily.
• It plays a critical role in maintaining the glomerular filtration barrier by stabilizing podocyte architecture and the interdigitating foot processes connected by the slit diaphragm.
• KIRREL1 functions as a signaling protein that can trans-activate the transcription factor AP-1 in the presence of TEC kinases, supporting podocyte homeostasis. Related references: PMID:31472902
• The protein is localized at the cell membrane, consistent with its role in cell-cell junction formation and signal transduction.
• It is abundantly and specifically expressed in podocytes of kidney glomeruli, with low or absent expression in other tissues.
• Annotations highlight alternative splicing, glycoprotein, phosphoprotein, and disease variant associations, underscoring its regulatory complexity and clinical relevance in nephrotic syndromes.
• The gene encodes a protein with a theoretical molecular weight of approximately 83.5 kDa, though experimental migration may vary.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (aa 17–200) of human KIRREL. For Western blot, a band is expected near 84 kDa; users should optimize loading amounts and validate in the relevant sample system.
• This polyclonal antibody is suitable for ELISA; consider pairing it with a monoclonal detection antibody to improve specificity in sandwich immunoassays.
• Documented cross-reactivity with mouse and rat allows application across common rodent models, but users should verify performance under their own experimental conditions.
• Polyclonal antibodies may exhibit lot-to-lot variability; include appropriate positive and negative controls in each run.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement, connecting diagnostic manufacturers with high-quality reagents like this anti-KIRREL polyclonal antibody for nephrology-focused assay development. Our portfolio spans validated antibody pairs, bulk monoclonals and polyclonals, ancillary reagents, and rare-target sourcing, all supported by end-to-end technical consultation. From early-stage biomarker discovery to clinical validation, CamelBio helps you bridge the gap between concept and clinic.