Anti-KIR3DS1 Polyclonal Antibody for WB, IHC-P, ELISA - Q14943

Core Product Specifications and Parameters

Biological Background: KIR3DS1 Function and Localization

• KIR3DS1 (Killer cell immunoglobulin-like receptor 3DS1, also known as CD158E2 or NKAT10) is an activating receptor expressed on natural killer (NK) cells that recognizes MHC class I molecules, specifically interacting with peptide-free HLA-F open conformers to trigger NK cell degranulation and antiviral cytokine production.
• As a member of the killer cell immunoglobulin-like receptor (KIR) family, KIR3DS1 contains immunoglobulin-like domains and is a transmembrane glycoprotein with a signal peptide.
• The receptor localizes to the cell membrane and is primarily found on NK cells and some T-cell lines; it is not expressed on B-lymphoblastoid cell lines or colon carcinoma cells.
• Key structural features include disulfide bonds, glycosylation, and multiple immunoglobulin domain repeats, as indicated by UniProt keywords.
• KIR3DS1 plays a critical role in innate immunity by mediating NK cell cytotoxicity and cytokine secretion upon engagement with its ligand, contributing to antiviral responses.

Experimental Guidance and Technical Tips

• For Western blot (WB): The expected molecular weight is 42 kDa. Use appropriate loading controls and validate antibody specificity with lysates from NK cell lines or tissues expressing KIR3DS1, such as spleen or peripheral blood mononuclear cells. Consider pre-adsorption controls if non-specific bands appear.
• For immunohistochemistry (IHC-P): Optimize antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and include positive tissue controls like human tonsil or spleen where NK cells are present. Cross-reactivity with mouse and rat suggests it may be suitable for detecting KIR3DS1 orthologs, but verify sequence homology and staining pattern.
• For ELISA: The antibody can be used for detection of recombinant or native KIR3DS1; validate assay conditions and consider using as a capture or detection antibody in sandwich ELISA formats with appropriate blocking steps.
• As a polyclonal antibody, batch-to-batch consistency should be verified; consider performing titration experiments to determine optimal working dilution for each application and sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, connecting diagnostic manufacturers and researchers with quality reagents like this KIR3DS1 antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials for immunoassays and immunology research. We support your pipeline from concept to clinic with reliable supply and technical insight.