Anti-KIR3DL1 Polyclonal Antibody for WB, ELISA - P43629

Core Product Specifications and Parameters

Biological Background: KIR3DL1 Function and Localization

• KIR3DL1 (Killer cell immunoglobulin-like receptor 3DL1) is an inhibitory receptor expressed on natural killer (NK) cells that specifically recognizes HLA-Bw4 alleles.
• Engagement of HLA-Bw4 inhibits NK cell-mediated cytotoxicity, preventing lysis of target cells.
• The protein is a transmembrane glycoprotein localized at the cell membrane, featuring an extracellular immunoglobulin domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM).
• KIR3DL1 belongs to the polymorphic killer cell immunoglobulin-like receptor family; alternative splicing generates isoforms such as KIR3DL1/S1.
• It is also known as CD158e, NKAT3, NKB1, and p70 NK receptor CL-2/CL-11.
• Post-translational modifications include disulfide bonds and glycosylation; a 3D structure is available.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular region (amino acids 22–222), suggesting the antibody may detect the receptor on the cell surface in native flow cytometry or immunofluorescence; however, validation is required.
• For Western blotting, a band near 49 kDa is expected; confirm specificity using NK cell lines (e.g., NKL, NK92) or transfected cells.
• For ELISA, the antibody can be paired with an appropriate detection antibody; test against recombinant KIR3DL1 protein and lysates from human or mouse tissues given cross-reactivity.
• As a polyclonal antibody, it may recognize multiple epitopes; consider potential batch-to-batch variability and validate in your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your raw material supply chain by offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Whether you are developing KIR3DL1-based diagnostic assays or require rare target sourcing, our integrated platform connects you with the materials you need—from concept to clinic.