Anti-KIR2DL4 Polyclonal Antibody for WB, ELISA - Q99706

Core Product Specifications and Parameters

Biological Background: KIR2DL4 Function and Localization

• KIR2DL4 functions as a receptor for non-classical MHC class Ib molecule HLA-G when complexed with B2M and nonamer self-peptide.
• In decidual natural killer (NK) cells, KIR2DL4 binds peptide-bound HLA-G-B2M, triggering a senescence-associated secretory phenotype that acts as a molecular switch to promote vascular remodeling and fetal growth during early pregnancy. Related references: PMID:16366734, PMID:23184984, PMID:29262349
• KIR2DL4 signaling contributes to balancing tolerance and antiviral immunity at the maternal-fetal interface by regulating effector functions of NK cells, CD8+ T cells, and B cells. Related references: PMID:10190900, PMID:16366734
• Upon ligand engagement, KIR2DL4 initiates signaling from endosomal compartments, leading to activation of PRKDC-XRCC5 and AKT1, and ultimately NF-kappa-B-dependent pro-inflammatory responses. Related references: PMID:20179272
• KIR2DL4 is localized to the cell membrane and early endosome membrane.
• It is expressed in decidual NK cells, innate lymphoid cell type I (ILC1), and a subset of peripheral NK cells. Related references: PMID:29262349, PMID:19304799
• Belongs to the killer cell immunoglobulin-like receptor (KIR) family and contains Ig-like domains, a transmembrane helix, and is subject to alternative splicing and glycosylation.

Experimental Guidance and Technical Tips

• The immunogen spans the extracellular domain (aa 22-242) of human KIR2DL4, potentially enabling recognition of both native and denatured protein; consider validating for WB and ELISA as listed.
• Predicted molecular weight is ~41 kDa; verify band specificity in appropriate positive controls (e.g., NK cell lines or decidual tissue lysates).
• Reported cross-reactivity with mouse and rat suggests epitope conservation; however, sequence alignment and experimental validation in these species are recommended.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop IVD raw-material supplier, CamelBio bridges the gap between concept and clinic for diagnostic manufacturers and researchers. This KIR2DL4 polyclonal antibody supports studies in reproductive immunology and NK cell biology. In addition to this reagent, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target sourcing to accelerate your assay development and biomarker discovery workflows.