Anti-KIR2DL4 Monoclonal Antibody for WB, FC, ELISA - Q99706

Core Product Specifications and Parameters

Biological Background: KIR2DL4 Function and Localization

• KIR2DL4 is a killer cell immunoglobulin-like receptor encoded by the KIR2DL4 gene, belonging to the KIR family of membrane glycoproteins involved in natural killer cell regulation.
• It functions as a receptor for non-classical MHC class I molecule HLA-G, specifically recognizing HLA-G in complex with beta-2 microglobulin (B2M) and a nonamer self-peptide.
• In decidual natural killer (NK) cells, binding of peptide-bound HLA-G-B2M complex triggers NK cell senescence-associated secretory phenotype, a molecular switch promoting vascular remodeling and fetal growth during early pregnancy. Related references: PMID:16366734 PMID:23184984 PMID:29262349
• KIR2DL4 plays a role in balancing tolerance and antiviral immunity at the maternal-fetal interface by modulating the effector functions of NK cells, CD8+ T cells, and B cells. Related references: PMID:10190900 PMID:16366734
• Upon interaction with peptide-bound HLA-G-B2M, KIR2DL4 initiates signaling from the early endosome compartment, leading to activation of PRKDC-XRCC5, AKT1, and NF-kappa-B-dependent pro-inflammatory responses. Related references: PMID:20179272
• The protein localizes to the cell membrane and early endosome membrane, consistent with its role in endosomal signaling.
• It is predominantly expressed in decidual NK cells and innate lymphoid cell type 1 (ILC1), with a subset found in peripheral NK cells. Related references: PMID:29262349 PMID:19304799
• Structural features include multiple immunoglobulin domains, disulfide bonds, glycosylation sites, a transmembrane helix, and a signal peptide, classifying it as a type I transmembrane receptor.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 24-242), suggesting the antibody may recognize the native receptor on live cells, making it suitable for flow cytometry.
• For Western blot, expect a band around 41 kDa. Due to predicted disulfide bonds, consider using reducing conditions to ensure consistent migration.
• In flow cytometry, validate reactivity using KIR2DL4-expressing cell lines or primary decidual NK cells, as expression is restricted to specific NK cell subsets.
• As the antibody is a recombinant rabbit monoclonal, lot-to-lot consistency is expected; however, titration and optimization are recommended for each assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material sourcing, offering validated antibody pairs, bulk reagents, and tailored procurement solutions for immunology and immuno-oncology targets like KIR2DL4. Whether you need this anti-KIR2DL4 monoclonal antibody for assay development or seek complementary raw materials for NK cell-related diagnostic kits, we provide reliable, research-grade supplies. Let us support your pipeline from concept to clinic with efficient access to rare and standard immunological reagents.