Anti-KIR2DL3 Polyclonal Antibody for WB, IF/ICC, ELISA - P43628

Core Product Specifications and Parameters

Biological Background: KIR2DL3 Function and Localization

• KIR2DL3 encodes a killer cell immunoglobulin-like receptor (KIR) that belongs to the KIR family of inhibitory receptors expressed on natural killer (NK) cells.
• It functions as an inhibitory receptor by binding specific HLA-C alleles (HLA-Cw1, HLA-Cw3, HLA-Cw7), delivering signals that prevent NK cell-mediated cytotoxicity and lysis of target cells.
• The protein is a transmembrane receptor with two immunoglobulin-like domains, an ITIM motif in its cytoplasmic tail, and undergoes alternative splicing, resulting in multiple isoforms.
• KIR2DL3 is localized to the cell membrane of NK cells and possibly other lymphocyte subsets.
• Post-translational modifications include disulfide bond formation and N-linked glycosylation, which are critical for receptor structure and ligand binding.
• KIR2DL3 is part of a diverse KIR gene cluster on chromosome 19q13.4; its expression contributes to NK cell education and self-tolerance.

Experimental Guidance and Technical Tips

• The immunogen covers the extracellular domain (aa 22–245), so the antibody is expected to recognize the native receptor; consider optimizing epitope retrieval for formalin-fixed samples.
• For Western blot (WB), a band near 38 kDa is anticipated; validate in relevant NK cell lines or primary cells under reducing conditions.
• For immunofluorescence/ICC, fixation and permeabilization conditions should be empirically tested; paraformaldehyde or methanol can be suitable starting points.
• For ELISA, the antibody may be used to detect recombinant KIR2DL3; include negative controls with irrelevant IgG to assess specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated antibodies, bulk ancillary reagents, and rare-target sourcing for immune checkpoint and natural killer cell biology research. With this KIR2DL3 polyclonal antibody, we support your NK cell receptor studies, from assay development to clinical translation, backed by reliable supply and technical consultation.