Anti-Kininogen 1 (KNG1) Rabbit Polyclonal Antibody for WB - P01042

Core Product Specifications and Parameters

Biological Background: Kininogen 1 Function and Localization

• Kininogen 1, also known as alpha-2-thiol proteinase inhibitor or Fitzgerald factor, is a secreted protein that exists as high molecular weight (HMW) and low molecular weight (LMW) isoforms through alternative splicing.
• As an inhibitor of thiol proteases, kininogen 1 modulates proteolytic activity in plasma and plays a crucial role in blood coagulation by positioning prekallikrein and factor XI near factor XII.
• The HMW kininogen isoform inhibits thrombin- and plasmin-induced thrombocyte aggregation, while the LMW isoform inhibits thrombocyte aggregation but is not involved in blood clotting.
• The active peptide bradykinin is liberated from HMW kininogen and acts as a potent vasodilator with multiple physiological effects, including smooth muscle contraction, hypotension, natriuresis, diuresis, decreased blood glucose, inflammation mediation, and cardioprotection.
• Kininogen 1 is predominantly secreted into the extracellular space and plasma; it is detected at elevated levels in malignant ovarian, colon, and breast carcinomas.
• Post-translational modifications include glycosylation, hydroxylation, phosphorylation, disulfide bond formation, and pyrrolidone carboxylic acid formation, which may regulate its stability and function.
• The protein has been associated with hemostasis, inflammatory response, vasoactivity, and disease variants linked to coagulation disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 148-427 of human KNG1, covering regions common to both isoforms; verify antibody reactivity with recombinant HMW and LMW kininogen if isoform-specific detection is required.
• Validate the antibody in relevant sample types, such as human plasma or serum, or cell lines known to express KNG1 (e.g., liver-derived cells).
• For Western blot, use appropriate reducing conditions to resolve the ~72 kDa full-length protein; note that some truncated forms may appear due to protease cleavage.
• In ELISA, consider pairing with a capture antibody targeting a distinct epitope to avoid competition and enhance signal.
• Cross-reactivity with mouse and rat has been demonstrated, but confirm in your specific experimental system as sequence divergence may affect binding.

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