Anti-Kininogen 1 (KNG1) Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P01042

Core Product Specifications and Parameters

Biological Background: Kininogen 1 Function and Localization

• Kininogen-1 (KNG1) is a secreted glycoprotein that serves as a precursor to bradykinin, a potent vasoactive peptide, and acts as a thiol protease inhibitor.
• It exists in two alternatively spliced isoforms: high molecular weight kininogen (HMWK) and low molecular weight kininogen (LMWK). HMWK plays a critical role in the intrinsic blood coagulation pathway by positioning prekallikrein and factor XI on factor XII, whereas LMWK is not involved in clotting.
• Upon cleavage by plasma kallikrein, HMWK releases bradykinin, which induces vasodilation, hypotension, increased vascular permeability, and pain; it also stimulates the release of other inflammatory mediators.
• Bradykinin also exhibits cardioprotective effects, directly and via endothelium-derived relaxing factor, and influences smooth muscle contraction, natriuresis, and glucose metabolism.
• Both isoforms inhibit thrombin- and plasmin-induced platelet aggregation, contributing to hemostatic regulation.
• KNG1 is secreted into the extracellular space and plasma; T-kinin, a related peptide, has been detected in malignant ovarian, colon, and breast carcinomas but not in benign tumors.
• Post-translational modifications include hydroxylation, phosphorylation, and disulfide bond formation; it is also a pyrrolidone carboxylic acid and glycoprotein.
• Mutations in KNG1 are associated with hereditary angioedema type 6 (HAE6), a disorder characterized by recurrent episodes of severe swelling.

Experimental Guidance and Technical Tips

• For Western blotting, a starting dilution of 1:500–1:2000 is commonly used for polyclonal antibodies; optimize based on your sample and detection system. The expected molecular weight of KNG1 is approximately 72 kDa, though glycosylation may result in a slightly higher apparent mass.
• In immunofluorescence/immunocytochemistry, consider using a permeabilization step to detect intracellular kininogen, as the protein is secreted and may be localized in vesicles or on the cell surface.
• For ELISA, this antibody can be employed as a capture or detection reagent; pair it with a suitable matched antibody for sandwich ELISA or use it to directly measure kininogen levels in plasma or conditioned media.
• Given the predicted cross-reactivity with mouse and rat, verify reactivity with your specific species and tissue model, as sequence homology suggests recognition but not guaranteed binding.
• The immunogen spans residues 148-427, which includes the kinin domain; thus the antibody likely recognizes both HMWK and LMWK isoforms.
• As with any polyclonal antibody, lot-to-lot variability may occur; extensive validation in your experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with premium raw materials for immunoassay development. This Kininogen 1 (KNG1) polyclonal antibody is part of a comprehensive portfolio that includes matched antibody pairs, recombinant proteins, and ancillary reagents to support coagulation and inflammation research. For bulk orders or customized sourcing solutions, contact our specialists.