Anti-Kinesin light chain 1 (KLC1) Polyclonal Antibody for WB, IHC-P, ELISA - Q07866

Core Product Specifications and Parameters

Biological Background: Kinesin Light Chain 1 (KLC1) Function and Localization

• Kinesin light chain 1 (KLC1), also known as KLC, KNS2, or KNS2A, is a component of the kinesin motor protein complex involved in intracellular transport.
• KLC1 functions in microtubule-dependent organelle transport, as demonstrated by studies showing its role in cargo movement (PubMed:21385839). Related references: PMID:21385839
• The light chain is thought to couple cargo to the kinesin heavy chain or modulate its ATPase activity, facilitating efficient motor function (By similarity).
• KLC1 is localized to the cytoplasm, associating with cytoskeletal structures, cytoplasmic vesicles, and growth cones of neuronal cells.
• It is highly expressed in the brain and spine, consistent with its critical role in neuronal transport and synapse function.
• The protein contains multiple TPR repeats and coiled-coil regions, and is subject to alternative splicing and phosphorylation, which may regulate its activity and interactions.
• KLC1 has been implicated in host-virus interactions, suggesting a role in viral trafficking within cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 261–560 of human KLC1 (NP_005543.2), a region likely recognizing multiple epitopes due to the polyclonal nature of the antibody.
• For Western blot (WB), consider using lysates from brain or neuronal cell lines where KLC1 is abundant; the predicted molecular weight is ~65 kDa, but note potential isoforms or post-translational modifications.
• For immunohistochemistry (IHC-P), validate in central nervous system tissues (e.g., brain, spinal cord) where robust expression is expected.
• In ELISA, the antibody may be used for direct detection; titrate and validate using recombinant KLC1 or purified protein to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, including validated antibodies like this KLC1 pAb, to support research in motor protein biology, intracellular trafficking, and neurobiology. Our portfolio includes monoclonal and polyclonal antibodies, antibody pairs, and bulk ancillary reagents, backed by technical consulting to streamline your assay development.