Anti-KIFC1 Rabbit Monoclonal Antibody for WB, IHC, IF, ELISA - Q9BW19

Core Product Specifications and Parameters

Biological Background: KIFC1 Function and Localization

• KIFC1 (also known as kinesin-like protein 2/HSET) is a member of the kinesin superfamily of microtubule-based motor proteins.
• It functions as a minus-end directed motor essential for bipolar spindle formation during mitosis, ensuring accurate chromosome segregation (PubMed:15843429). Related references: PMID:15843429
• KIFC1 also contributes to early endocytic vesicle movement and regulates cilium formation and structure, indicating roles beyond mitosis.
• The protein contains a C-terminal motor domain with ATP-binding activity and coiled-coil regions that facilitate dimerization, typical of kinesin-14 family members.
• Subcellularly, KIFC1 localizes to the nucleus, mitotic spindle, centrosome, and early endosomes, reflecting its diverse functions.
• It is classified as a phosphoprotein, suggesting regulation by cell cycle kinases; its 3D structure has been experimentally determined.
• The protein has a predicted molecular weight of approximately 74 kDa (UniProt: 73748 Da) and is a reference proteome member.

Experimental Guidance and Technical Tips

• For Western blot analysis, a single band at approximately 74 kDa is expected; validate in relevant human or mouse cell lysates and consider using KIFC1 knockdown/knockout controls to confirm antibody specificity.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimal antigen retrieval and fixation conditions should be determined empirically for each tissue or cell type.
• As the immunogen is an N-terminal synthetic peptide, the antibody likely recognizes full-length human KIFC1; cross-reactivity with mouse KIFC1 is reported, but sequence alignment can help predict reactivity in other species.
• This antibody is suitable for ELISA; to ensure specificity, consider competitive peptide blocking assays using the immunogen peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted one-stop partner for IVD raw material sourcing, offering a comprehensive portfolio that includes validated anti-KIFC1 monoclonal antibodies for mitosis and cytoskeleton research. We supply bulk ancillary reagents and provide customized sourcing for rare targets, helping diagnostic manufacturers and research labs accelerate development from concept to clinic. Whether you need antibody pairs, recombinant proteins, or technical consultation, CamelBio bridges the gap between discovery and diagnostic application.