Anti-KIFC1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9BW19

Core Product Specifications and Parameters

Biological Background: KIFC1 Function and Localization

• KIFC1 (also known as HSET or KNSL2) is a kinesin-like protein belonging to the kinesin-14 family of motor proteins.
• It functions as a minus end-directed microtubule-dependent motor essential for bipolar spindle formation during mitosis (PubMed:15843429).
  Related references:
  PMID:15843429
• The protein localizes to multiple cellular compartments including the nucleus, cytoplasm, centrosomes, mitotic spindle, and early endosomes.
• KIFC1 contains characteristic coiled-coil domains common to kinesins and possesses ATP-binding and nucleotide-binding activities.
• It undergoes phosphorylation, which may regulate its motor activity during cell division.
• Beyond mitosis, KIFC1 contributes to cilium formation and structure, and may play a role in endocytic vesicle movement.
• The human KIFC1 gene encodes a protein with a predicted molecular weight of approximately 74 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 1–250 of human KIFC1, suggesting the antibody recognizes the N-terminal region. Consider this when designing epitope mapping or blocking experiments.
• The antibody is suitable for Western blot (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA, as indicated by the supplier. Optimize dilutions and conditions for each assay in the relevant sample system.
• For WB, the predicted molecular weight is 74 kDa. Use appropriate positive controls from human, mouse, or rat cell lines, as cross-reactivity is expected with these species.
• In IHC-P and IF/ICC, fixation and antigen retrieval methods should be validated; subcellular localization to the nucleus, centrosome, and spindle may require optimized sample preparation.
• Since KIFC1 is a motor protein involved in mitosis, synchronization of cells may enhance detection of spindle-localized protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes a streamlined one-stop channel for IVD raw materials, including validated antibodies targeting cell cycle regulators like KIFC1. Our portfolio encompasses polyclonal and monoclonal antibodies, ancillary reagents, and bulk sourcing solutions to support your assay development from concept to clinic. Reach out to our team for reliable sourcing of rare-target raw materials and dedicated technical consultation.