Anti-KIFAP3 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q92845

Core Product Specifications and Parameters

Biological Background: KIFAP3 Function and Localization

• KIFAP3, also known as Smg GDS-associated protein (SMAP), is a kinesin-associated protein involved in chromosome tethering to the spindle pole and chromosome movement during mitosis.
• It binds to the tail domain of the KIF3A/KIF3B heterodimer, forming a heterotrimeric KIF3 complex that may regulate the membrane binding of this motor complex (By similarity).
• The gene KIFAP3 (also designated KIF3AP) is mapped to human chromosome region and encodes a protein of approximately 91 kDa.
• According to UniProt annotations, the protein undergoes alternative splicing and is classified as a phosphoprotein; it contains repeat domains of unknown function.
• Subcellularly, KIFAP3 localizes to the mitotic spindle, consistent with its role in chromosome dynamics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–200) of human KIFAP3. This antibody likely recognizes full-length KIFAP3 and any N-terminal isoforms that retain this epitope.
• In Western blot, a band around 91 kDa is expected. Validate specificity using lysates from cell lines with known KIFAP3 expression and appropriate controls.
• For immunofluorescence/ICC, KIFAP3 localizes to the mitotic spindle. Optimize fixation and permeabilization conditions, as spindle-associated proteins can be sensitive to standard protocols.
• The antibody is reported to cross-react with mouse and rat KIFAP3. Given high sequence homology in the immunogen region, it is likely suitable for these species, but independent validation is recommended.
• For IP and ELISA applications, follow standard protocols and consider the polyclonal nature of the antibody in interpreting results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a streamlined pipeline from research-grade reagents to bulk diagnostic components. For researchers and manufacturers focused on chromosome dynamics and mitosis, we provide validated antibodies like this anti-KIFAP3 polyclonal antibody, along with matched antibody pairs, recombinant proteins, and ancillary reagents. Leverage our network to secure optimized, high-quality raw materials for your assay development and manufacturing needs.