Anti-KIFAP3 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q92845

Core Product Specifications and Parameters

Biological Background: KIFAP3 Function and Localization

• KIFAP3, also annotated as Smg GDS-associated protein (SMAP), is a kinesin-associated protein encoded by the KIFAP3 gene (aliases KIF3AP, SMAP).
• Functionally, KIFAP3 acts as an adaptor that binds the tail domain of the KIF3A/KIF3B heterodimer to form the heterotrimeric KIF3 motor complex, potentially regulating its attachment to intracellular membranes and cargo.
• The KIF3 complex is implicated in tethering chromosomes to the mitotic spindle pole and facilitating proper chromosome movement during cell division (by similarity).
• Alternative splicing of the KIFAP3 transcript generates multiple isoforms, increasing functional diversity.
• The protein is annotated as a phosphoprotein, suggesting regulation by phosphorylation in signaling or cell cycle contexts.
• The human KIFAP3 protein has a calculated molecular mass of approximately 91 kDa (UniProt), consistent with the observed ~91 kDa band on western blots.
• Keywords from UniProt highlight its classification under reference proteome and proteomics identification, confirming its broad detection in proteomic studies.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 675-792 of human KIFAP3) targets the C‑terminal region; verify reactivity if your target sequence differs in other species (e.g., mouse or rat).
• For western blot, expect a specific band at ~91 kDa under reducing conditions; validate in relevant cell lysates (e.g., HeLa, mouse brain).
• In immunofluorescence/ICC, mitotic cells may show spindle‑associated or chromosome‑associated staining; consider enriching mitotic populations with nocodazole and including isotype controls.
• The antibody has been validated for ELISA; for quantitative detection, include a standard curve with recombinant KIFAP3 protein if available.
• As a rabbit monoclonal antibody, ensure the secondary antibody is compatible and verify absence of cross‑reactivity with endogenous rabbit IgG in your sample type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with integrated sourcing of IVD raw materials. This KIFAP3 monoclonal antibody—ideal for studies on mitotic spindle assembly, chromosome segregation, and kinesin motor complexes—is part of our extensive catalog. We also offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, helping to streamline your assay development from concept to clinic.