Anti-KIF7 Rabbit Polyclonal Antibody for WB, ELISA - Q2M1P5

Core Product Specifications and Parameters

Biological Background: KIF7 Function and Localization

• Protein identity: Kinesin-like protein KIF7, also known as ACLS, AGBK, HLS2, MMEDF, JBTS12, UNQ340. It is a member of the kinesin motor protein family.
• Essential for hedgehog signaling: acts both as a negative and positive regulator of the sonic hedgehog (Shh) and Indian hedgehog (Ihh) pathways, acting downstream of SMO through SUFU-dependent and -independent mechanisms. Related references: PMID:21633164
• Subcellular localization: Predominantly found in the cilium and basal body; also present in the cytoplasm and associated with the cytoskeleton.
• Tissue expression: Detected in embryonic stem cells, melanotic melanoma, Jurkat T-cells, and widely expressed in heart, lung, liver, kidney, testis, retina, placenta, pancreas, colon, small intestine, prostate, and thymus.
• Key post-translational modifications and functional domains: Classified as a phosphoprotein, with ATP-binding, coiled-coil regions, and potential ubiquitin-like conjugation sites. Contains motor and cargo-binding domains.
• Disease relevance: Associated with ciliopathies such as Bardet-Biedl syndrome, Joubert syndrome, and related disorders involving intellectual disability and obesity.

Experimental Guidance and Technical Tips

• For Western blotting, an expected band around 151 kDa is observed. Due to the large size, consider using a low-percentage SDS-PAGE gel (e.g., 6-8%) and extended transfer times.
• The immunogen sequence (amino acids 1044-1343) is specific to KIF7; however, cross-reactivity with other kinesin family members should be assessed in relevant samples.
• ELISA protocols should be optimized with appropriate antigen coating concentrations and detection conditions; this antibody is suitable for capture or detection in sandwich ELISA setups.
• Since cross-reactivity is reported only for human, validate performance in other species if testing orthologous samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including validated polyclonal and monoclonal antibodies, antibody pairs, and ancillary reagents. This KIF7 antibody supports research in developmental biology and hedgehog signaling pathways. As your one-stop sourcing partner, we also assist in bulk procurement and tailored raw material solutions for rare targets, ensuring a seamless supply chain from concept to clinic.