Anti-KIF5B Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P33176

Core Product Specifications and Parameters

Biological Background: KIF5B Function and Localization

• KIF5B encodes the heavy chain of kinesin-1, a plus-end-directed microtubule motor protein essential for intracellular transport.
• The protein, also known as conventional kinesin heavy chain or ubiquitous kinesin heavy chain (UKHC), mediates the movement of organelles such as mitochondria and lysosomes along microtubules.
• KIF5B regulates centrosome and nuclear positioning during mitotic entry. In the G2 phase, it drives the separation of nuclei and centrosomes by antagonizing dynein function in a BICD2-dependent manner. Related references: PMID:20386726
• In neurons, KIF5B is required for anterograde axonal transport of MAPK8IP3/JIP3, which is crucial for axon elongation.
• Through interactions with PLEKHM2 and ARL8B, KIF5B directs lysosome movement toward microtubule plus ends.
• KIF5B contributes to natural killer (NK) cell-mediated cytotoxicity by polarizing cytolytic granules and microtubule-organizing centers toward the immune synapse. Related references: PMID:24088571
• Subcellularly, KIF5B localizes to the cytoplasm, cytoskeleton, cytolytic granule membrane, and lysosome membrane, consistent with its transport roles.
• Post-translational modifications including acetylation, phosphorylation, methylation, and ubiquitination may regulate its motor activity and cargo binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 650–770 in the C-terminal region of human KIF5B, which is present in most isoforms and conserved across human, mouse, and rat.
• For Western blot (WB), a band near 110 kDa is expected; ensure proper sample homogenization and use of protease inhibitors.
• In IHC-P and IF/ICC, antigen retrieval and permeabilization could be necessary; fixation and blocking conditions should be optimized for the target tissue or cells.
• For ELISA, the antibody might be used as a capture or detection reagent, but pair compatibility with target protein standards should be validated.
• As a polyclonal antibody, batch-to-batch variability is possible; performance in specific assay conditions must be verified.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our portfolio supports cytoskeletal and motor protein research, including kinesin family members like KIF5B, to accelerate your diagnostic and assay development projects. We provide comprehensive technical support to bridge your raw material needs from concept to clinic.