Anti-KIF5B Monoclonal Antibody for WB, IF-P, ELISA - P33176

Core Product Specifications and Parameters

Biological Background: KIF5B Function and Localization

• Encodes kinesin-1 heavy chain, also known as conventional kinesin heavy chain or ubiquitous kinesin heavy chain (UKHC), a member of the kinesin motor protein superfamily.
• Functions as a microtubule-dependent motor protein required for normal distribution of mitochondria and lysosomes, and can induce neurite-like membrane protrusions in non-neuronal cells.
• Regulates centrosome and nuclear positioning during mitotic entry by antagonizing dynein function in a BICD2-dependent manner. Related references: PMID:20386726
• Required for anterograde axonal transportation of MAPK8IP3/JIP3, which is essential for axon elongation, highlighting its role in neuronal development.
• Directs lysosome movement toward microtubule plus ends through binding to PLEKHM2 and ARL8B, linking kinesin-1 to lysosomal trafficking.
• Involved in NK cell-mediated cytotoxicity by driving polarization of cytolytic granules and microtubule-organizing centers toward the immune synapse. Related references: PMID:24088571
• Localizes to cytoplasm, cytoskeleton, cytolytic granule membrane, and lysosome membrane, consistent with its diverse cellular transport roles.
• Classified as an ATP-binding, coiled-coil, motor protein with documented acetylation, methylation, phosphorylation, and ubiquitination modifications.

Experimental Guidance and Technical Tips

• The immunogen (N-terminal amino acids 1-100) targets a region conserved across human, mouse, and rat, supporting the observed cross-reactivity; validate specificity in your target sample system and application.
• For Western blotting, the predicted molecular weight is ~110 kDa. Optimize antibody dilution and load appropriate positive controls expressing KIF5B (e.g., HeLa, brain lysates).
• This antibody is suitable for immunofluorescence of paraformaldehyde-fixed samples (IF-P); ensure proper fixation, permeabilization, and blocking steps.
• For ELISA, consider pairing with a detection antibody against a non-overlapping epitope; titrate to determine optimal working concentrations.
• Performance in untested applications (e.g., IHC, flow cytometry) should be verified by end users using appropriate experimental setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a one-stop sourcing solution for IVD raw materials, empowering diagnostic manufacturers and researchers with validated antibodies, bulk ancillary reagents, and customized procurement services. This Anti-KIF5B antibody supports investigations into intracellular transport, motor protein biology, and related pathologies. Contact us to learn how CamelBio can streamline your supply chain from concept to clinic with reliable, high-quality reagents.