Anti-KIF5A Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q12840

Core Product Specifications and Parameters

Biological Background: KIF5A Function and Localization

• KIF5A is a neuron-specific kinesin heavy chain, also known as Neuronal kinesin heavy chain (NKHC), encoded by the KIF5A (NKHC1) gene.
• It functions as a microtubule-dependent motor protein essential for slow axonal transport of neurofilament proteins (NFH, NFM, NFL).
• KIF5A can induce the formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner.
• The ZFYVE27-KIF5A complex is involved in vesicular transport of several proteins, including VAPA, VAPB, SURF4, RAB11A, RAB11B, and RTN3, in neurons.
• It is required for anterograde axonal transportation of MAPK8IP3/JIP3, which is essential for MAPK8IP3/JIP3 function in axon elongation.
• Subcellularly, KIF5A localizes to the cytoplasm, perinuclear region, cytoskeleton, and specifically in the perikaryon of neurons.
• KIF5A is distributed throughout the central nervous system but is highly enriched in subsets of neurons.
• Keywords associated with KIF5A include its role in neurodegenerative diseases, with disease variants linked to amyotrophic lateral sclerosis, hereditary spastic paraplegia, and epilepsy; it undergoes post-translational modifications such as acetylation and phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (amino acids 933-1032) of human KIF5A; this region is relatively conserved, supporting the observed cross-reactivity with mouse and rat.
• For Western blot, the predicted molecular weight is approximately 117 kDa; optimize gel percentage and transfer conditions for high-molecular-weight proteins, and consider using a loading control of similar size.
• For immunofluorescence/ICC, validate fixation and permeabilization protocols for your neuronal cell type, and co-staining with a cytoskeletal marker (e.g., tubulin) may confirm subcellular localization.
• ELISA applications can be used for detecting KIF5A, but ensure proper controls and standard curves are established; note that KIF5A expression is neuron-specific.

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CamelBio is a one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers, labs, and research institutes with reliable access to reagents like this KIF5A antibody for neurobiology and neurodegenerative disease research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your diagnostic or research projects. Contact us to explore how our integrated supply chain can support your next development.