Anti-KIF4A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95239

Core Product Specifications and Parameters

Biological Background: KIF4A Function and Localization

• KIF4A (Chromokinesin-A) is a member of the kinesin superfamily of motor proteins, containing ATP-binding and microtubule-binding domains, and is essential for mitotic chromosome segregation.
• KIF4A is an iron-sulfur (Fe-S) cluster-binding motor protein critical for chromosome segregation during mitosis. Related references: PMID:29848660
• During the metaphase-to-anaphase transition, KIF4A translocates PRC1 to the plus ends of interdigitating spindle microtubules, promoting central spindle midzone and midbody formation and successful cytokinesis. Related references: PMID:15297875, PMID:15625105
• May also participate in mitotic chromosomal positioning and bipolar spindle stabilization.
• Located in the nucleus matrix, cytoplasm, mitotic spindle, midbody, and chromosomes during cell division.
• Highly expressed in hematopoietic tissues including fetal liver, spleen, thymus, and adult thymus and bone marrow; lower expression in heart, testis, kidney, colon, and lung.
• Post-translationally modified by phosphorylation and ubiquitination (isopeptide bond and Ubl conjugation), regulating its activity and stability.
• Associated with intellectual disability (MRX100, XLID100) and exhibits alternative splicing, leading to multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 870–1080) lies in the C‑terminal region, which may provide high specificity by avoiding conserved motor domains. Validate reactivity in human, mouse, and rat samples.
• For Western blot, expect a band at approximately 140 kDa; use mitotic cell lysates as positive controls and confirm with knockdown or overexpression.
• For IHC‑P and IF/ICC, optimize antigen retrieval and permeabilization; strong nuclear and spindle staining is expected in dividing cells.
• ELISA performance should be optimized by checkerboard titrations to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, supporting research and diagnostic development from early discovery to clinical application. This anti‑KIF4A antibody is ideal for mitosis and cell division studies, and CamelBio can supply validated antibody pairs, optimized polyclonal/monoclonal antibodies, bulk ancillary reagents, and assist in sourcing rare targets to streamline your workflow.