Anti-KIF3A Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y496

Core Product Specifications and Parameters

Biological Background: KIF3A Function and Localization

• KIF3A is a kinesin-like motor protein, also designated microtubule plus end-directed kinesin motor 3A.
• It exhibits ATP-dependent plus end-directed microtubule sliding activity, functioning as an anterograde translocator for membranous organelles.
• KIF3A plays a critical role in primary cilia formation by mediating the transport of proteins, such as smoothened (SMO), into the cilium in response to hedgehog morphogens.
• The protein is involved in centriole cohesion and the organization of subdistal appendages, where it recruits DCTN1 to the centriole.
• It is required for ciliary basal feet formation and the anchoring of microtubules to the mother centriole.
• Subcellular localization includes the cytoplasm, cytoskeleton, cilium, and centrosome/centriole, consistent with its roles in ciliary and centrosomal dynamics.
• Keywords associated with KIF3A include ATP-binding, cilium biogenesis/degradation, coiled coil, microtubule motor activity, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 600–699, a region within the C-terminal stalk/tail domain; this design may provide specificity for KIF3A over other kinesin family members.
• For Western blot (WB), an expected band near 80 kDa should be detected in lysates from human, mouse, or rat cells. Validate expression in the relevant sample system using positive and negative controls.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider antigen retrieval methods and permeabilization protocols optimal for the target tissue. Nuclear and ciliary staining patterns may be observed, reflecting localization.
• In ELISA, use the peptide immunogen as a positive capture control to verify antibody binding; assay conditions should be empirically determined.
• As a rabbit monoclonal antibody, it offers consistent batch-to-batch reproducibility for quantitative applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with streamlined access to a comprehensive portfolio of IVD raw materials. This anti-KIF3A antibody supports studies in cytoskeletal dynamics, ciliogenesis, and centrosome biology. Beyond high-quality monoclonal antibodies, CamelBio offers validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing to accelerate your assay development.