Anti-KIF3A Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q9Y496

Core Product Specifications and Parameters

Biological Background: KIF3A Function and Localization

• KIF3A is a microtubule plus-end-directed motor protein (kinesin) that drives anterograde transport of membranous organelles.
• Plays a critical role in primary cilium formation by mediating protein transport into the cilium, including smoothened (SMO) in hedgehog signaling.
• Functions in centriole cohesion and organization, regulating subdistal appendage formation via DCTN1 recruitment.
• Required for ciliary basal feet formation and microtubule anchoring to the mother centriole.
• Localizes to the cytoplasm, cytoskeleton, centriole, and ciliary axoneme.
• Contains coiled-coil domains for dimerization and an ATP-binding motor domain for microtubule-based motility.
• Belongs to the kinesin motor protein family and is subject to phosphorylation.

Experimental Guidance and Technical Tips

• For Western blot (WB), the expected molecular weight of KIF3A is approximately 80 kDa; be sure to include appropriate molecular weight markers.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), consider optimizing antigen retrieval and permeabilization protocols to detect centrosomal and ciliary staining.
• The immunogen corresponds to amino acids 430-699 of human KIF3A, a region that may influence antibody specificity; validate in your target species and tissue.
• When performing co-immunoprecipitation (IP), note that KIF3A interacts with other kinesin subunits (e.g., KIF3B) and accessory proteins; confirm complex formation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-KIF3A polyclonal antibody for studying kinesin motor functions. Beyond individual reagents, we provide one-stop sourcing for antibody pairs, bulk ancillary reagents, and custom raw-material solutions to support your diagnostic development from concept to clinic. Contact us for optimized monoclonal/polyclonal options and technical consultation for your KIF3A-related projects.