Anti-KIF2C/MCAK Rabbit Monoclonal Antibody for WB, ELISA - Q99661

Core Product Specifications and Parameters

Biological Background: KIF2C/MCAK Function and Localization

• KIF2C, also known as mitotic centromere-associated kinesin (MCAK) or kinesin-like protein 6, is a member of the kinesin-13 family and exists as multiple isoforms via alternative splicing; isoform 2 is testis-specific.
• In complex with KIF18B, KIF2C constitutes the major microtubule plus-end depolymerizing activity in mitotic cells. Related references: PMID:21820309
• KIF2C regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis. Related references: PMID:19060894
• KIF2C plays a role in chromosome congression and is required for the lateral to end-on conversion of chromosome-microtubule attachments. Related references: PMID:23891108
• Subcellularly, KIF2C localizes to the cytoplasm, cytoskeleton, nucleus, centromere, and kinetochore, consistent with its dynamic mitotic functions.
• Tissue expression is highest in thymus and testis, low in small intestine, colon, and placenta, and undetectable in most other tissues; the testis-specific isoform 2 is restricted to testis.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination; the protein contains ATP‑binding, coiled‑coil, and microtubule‑binding motor domains. Keywords highlight its involvement in cell cycle, mitosis, chromosome partition, and centromere/kinetochore dynamics.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N‑terminal region (aa 1–100) of human KIF2C; consider epitope location when designing detection systems or validation strategies.
• For western blotting, use reducing/denaturing conditions; load sufficient protein and validate with positive (e.g., thymus, testis lysates) and negative controls. The predicted molecular weight is ~81 kDa (UniProt: 81.3 kDa), but observed mobility may vary; confirm specificity with knockdown/knockout models if possible.
• For ELISA, optimize coating and detection antibody concentrations; the antibody can be used for direct or indirect detection of KIF2C in cell lysates or recombinant protein preparations.
• As a rabbit monoclonal, the antibody is expected to show high affinity; still, rule out potential cross‑reactivity with other kinesin‑13 family members by sequence alignment and empirical testing.
• Because KIF2C is essential for mitosis, consider using this antibody in cell cycle studies, especially to analyze kinetochore‑microtubule dynamics and chromosome segregation.

CamelBio: Your One-Stop Sourcing Bridge

As a one‑stop IVD raw‑material sourcing partner, CamelBio supports diagnostic manufacturers and research institutions with reliable access to high‑quality antibodies and ancillary reagents. This anti‑KIF2C/MCAK product is part of our portfolio targeting cell cycle and mitosis research, and we can assist with sourcing validated antibody pairs, bulk monoclonal/polyclonal antibodies, and rare‑target reagents tailored to your assay development needs. Contact us for customized procurement solutions that span the entire journey from concept to clinic.