Anti-KIF2A Polyclonal Antibody for WB and ELISA - O00139

Core Product Specifications and Parameters

Biological Background: KIF2A Function and Localization

• KIF2A (Kinesin-like protein KIF2A), also known as Kinesin-2 (hK2) or HK2, is a plus end-directed microtubule-dependent motor protein essential for normal brain development.
• It regulates microtubule dynamics during axonal growth and is crucial for mitotic progression, chromosome congression at the metaphase plate, and normal spindle dynamics.
• KIF2A possesses microtubule depolymerization activity, promoting spindle turnover and contributing to the formation of bipolar mitotic spindles.
• Subcellularly, KIF2A localizes to the cytoplasm, cytoskeleton, microtubule organizing center (centrosome), and spindle poles during cell division.
• Post-translational modifications include acetylation and phosphorylation (as indicated by UniProt keywords), which may regulate its activity and interactions.
• Functional keywords highlight its roles in ATP binding, cell cycle, cell division, mitosis, developmental processes, neurogenesis, and differentiation; disease associations include lissencephaly.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 30–220 of human KIF2A (NP_004511.2). For species other than human, mouse, or rat, verify sequence homology to ensure cross-reactivity.
• For Western blot, the predicted molecular weight of KIF2A is approximately 80 kDa; use appropriate molecular weight markers and validate detection in your sample system.
• ELISA applications may require optimization of coating antigen and antibody concentrations; recombinant protein or peptide can serve as positive controls.
• Given KIF2A's role in mitosis and neuronal development, consider testing in cell lines or tissues enriched for these processes (e.g., dividing cells, brain extracts).
• Note that this is a polyclonal antibody; batch-to-batch consistency should be verified in critical assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering validated antibody pairs, bulk ancillary reagents, and rare target sourcing for mitosis and neuroscience research. For your KIF2A-related projects, we ensure reliable supply of polyclonal and monoclonal antibodies, along with customized support to accelerate your diagnostic development.