Anti-KIF23 Rabbit Monoclonal Antibody for WB, IP, ELISA - Q02241

Core Product Specifications and Parameters

Biological Background: KIF23 Function and Localization

• KIF23, also known as kinesin-like protein 5 (KNSL5) or mitotic kinesin-like protein 1 (MKLP1), is a plus-end-directed motor enzyme that moves antiparallel microtubules in vitro.
• It is a component of the centralspindlin complex, essential for cytokinesis by mediating Rho-dependent signaling required for myosin contractile ring formation during cell division.
• KIF23 is required for the localization of ECT2 to the central spindle, further facilitating cytokinesis.
• The protein localizes to the nucleus, cytoplasm, cytoskeleton (spindle), and midbody, with specific localization to the midbody ring during cytokinesis.
• It is widely expressed in human tissues.
• KIF23 undergoes alternative splicing and is subject to post-translational modifications, including phosphorylation and ubiquitination.
• Mutations in KIF23 are associated with congenital dyserythropoietic anemia and hereditary hemolytic anemia.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 801-960) of human KIF23, which may provide specific recognition of the full-length protein. Consider sequence alignment to assess cross-reactivity with other species or family members.
• For Western blotting, a band at approximately 110 kDa is expected. Use appropriate positive controls such as HeLa or HEK293 cell lysates, and verify the detected band against the predicted molecular weight.
• For immunoprecipitation, cross-reactivity with human and rat samples is anticipated. Optimize antibody concentration and incubation conditions for your specific experimental system.
• For ELISA, direct coating of recombinant KIF23 protein or peptide is recommended. Include appropriate negative controls to assess background binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to high-quality IVD raw materials. This Anti-KIF23 rabbit monoclonal antibody exemplifies our commitment to providing validated reagents for cytokinesis and cell division research. Beyond individual antibodies, we supply optimized antibody pairs, bulk ancillary reagents, and specialty raw materials, acting as your comprehensive sourcing partner from discovery through clinical application.