Anti-KIF22 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q14807

Core Product Specifications and Parameters

Biological Background: KIF22 Function and Localization

• Kinesin-like protein KIF22, also designated KID, KNSL4, OBP-1, OBP-2, or SEMDJL2, is a member of the kinesin motor protein family and functions as a DNA-binding protein.
• It plays a critical role in mitotic and meiotic chromosome movements by cross-linking microtubules and DNA, aiding spindle formation. KIF22 contributes to the congression of laterally attached chromosomes in NDC80-depleted cells. Related references: PMID:25743205
• Subcellularly, KIF22 localizes to the nucleus, cytoplasm, and cytoskeleton, consistent with its dynamic role during cell division.
• Tissue expression is prominent in bone, cartilage, joint capsule, ligament, skin, and primary cultured chondrocytes, linking it to skeletal biology.
• Post-translational modifications include phosphorylation and ubiquitin-like conjugation (isopeptide bond), modulating its activity and stability.
• Mutations in KIF22 are associated with spondyloepimetaphyseal dysplasia with joint laxity (SEMDJL2), a form of dwarfism, underscoring its importance in skeletal development.
• The protein features conserved domains such as kinesin motor, coiled coil, and ATP/nucleotide-binding motifs, characteristic of mitotic motors.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 456–665 of human KIF22, targeting the C‑terminal region; this may recognize both full‑length and potential isoforms.
• For Western blotting, expect a major band at approximately 73 kDa; validate the detection in the appropriate cell or tissue lysate under denaturing conditions.
• In immunohistochemistry (paraffin), consider heat‑induced antigen retrieval and titrate the antibody to achieve optimal specific staining with minimal background.
• Cross‑reactivity with mouse enables studies in mouse models; however, preliminary testing is recommended to confirm reactivity in the chosen strain/tissue.
• ELISA protocols may involve coating with the recombinant immunogen or native protein; optimize the coating buffer and antibody dilution for the best signal‑to‑noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw‑material solutions, from validated antibodies and antigen pairs to bulk ancillary reagents, supporting diagnostic developers worldwide. This anti-KIF22 polyclonal antibody serves research into mitotic regulation and kinesin biology, with applications in oncology and genetic disease. Leverage our one‑stop sourcing for custom antibody development, rare‑target raw materials, and seamless transition from research to assay manufacturing.