Anti-KIF20A Polyclonal Antibody for WB, ELISA - O95235

Core Product Specifications and Parameters

Biological Background: KIF20A Function and Localization

• KIF20A, also known as mitotic kinesin-like protein 2 (MKlp2) or Rabkinesin-6, is a member of the kinesin superfamily of motor proteins.
• It functions as a microtubule plus-end-directed motor protein, essential for cytokinesis by recruiting the chromosome passenger complex (CPC) to the central spindle.
• Phosphorylation by PLK1 enables KIF20A to recruit PLK1 to the central spindle, a critical step in cell division.
• KIF20A interacts with GTP-bound RAB6A and RAB6B and may mediate retrograde transport of Golgi membranes and vesicles along microtubules.
• Subcellular localization includes the Golgi apparatus, cytoplasm, and spindle microtubules, consistent with its roles in mitosis and intracellular trafficking.
• The protein is subject to post-translational modifications, including phosphorylation and acetylation, and alternative splicing generates multiple isoforms.
• Mutations in KIF20A are associated with cardiomyopathy, highlighting its importance beyond cell division.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 591-890 of human KIF20A, a region likely exhibiting high immunogenicity and specificity. Consider using the antibody at appropriate dilutions for Western blot (WB) and ELISA.
• Cross-reactivity with mouse and rat has been reported, making this antibody suitable for studies in rodent models. Validate the antibody in the relevant sample system, as reactivity may vary.
• For WB, the predicted molecular weight is approximately 100 kDa; ensure proper gel electrophoresis and transfer conditions to detect this high molecular weight protein.
• Due to its role in mitosis, consider synchronizing cell cultures to enhance signal in proliferation-dependent assays.
• The antibody is shipped on ice bags to maintain stability; upon receipt, aliquot and store at recommended temperatures to avoid freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. We support diagnostic manufacturers and research institutes from concept to clinic. For researchers studying cell division, intracellular transport, or cancer biology, CamelBio provides reliable access to KIF20A antibodies and related tools, ensuring consistent quality and supply for your critical assays.