Anti-KIF19 Rabbit Polyclonal Antibody for WB, ELISA - Q2TAC6

Core Product Specifications and Parameters

Biological Background: KIF19 Function and Localization

• Kinesin-like protein KIF19 is a plus end-directed microtubule-dependent motor protein that mediates depolymerization of microtubules at ciliary tips, thereby regulating motile cilia length.

• KIF19 localizes to the cytoplasm, associates with the cytoskeleton, and specifically accumulates in the cilium, a specialized cell projection.

• According to UniProt annotation, KIF19 functions in microtubule dynamics and belongs to the kinesin motor protein family, containing ATP-binding and coiled-coil domains.

• Alternative splicing of KIF19 generates multiple isoforms; the canonical isoform has a calculated molecular weight of approximately 111 kDa.

• Key post-translational modifications and additional interaction data are not yet fully described, but proteomics studies suggest KIF19 is detectable in human cell lines.

• Tissue-specific expression of KIF19 has not been detailed, but its role in cilia suggests expression in ciliated tissues such as respiratory epithelium, ependyma, and oviduct.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1–300) of human KIF19, which includes the motor domain; this design may help detect multiple isoforms if the epitopes are conserved.

• For Western blotting, consider using a molecular weight marker around 111 kDa; validate antibody specificity using appropriate positive controls (e.g., ciliated cell lysates) and negative controls (e.g., non-ciliated cells or knockout/knockdown samples).

• In ELISA, the antibody may be used for antigen capture or detection; titrate antibody concentrations and include appropriate controls to ensure optimal signal-to-noise ratio.

• Since this is a polyclonal antibody, lot-to-lot variability is possible; we recommend standardizing with the same lot for reproducible experiments and validating cross-reactivity in your specific model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with streamlined access to a wide array of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. For researchers studying cilia biology, microtubule dynamics, or kinesin motor proteins, we can support your workflow with KIF19-related antibodies and complementary reagents. Connect with us to explore how our one-stop supply capabilities can accelerate your project from concept to clinic.