Anti-KIF17 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9P2E2

Core Product Specifications and Parameters

Biological Background: KIF17 Function and Localization

• KIF17 is a kinesin-like motor protein belonging to the kinesin superfamily, with an N-terminal motor domain that binds microtubules and hydrolyzes ATP.
• Alternative names include KIF3X, KLP-2, OSM-3, and KIF17B, reflecting homology to other kinesin family members.
• KIF17 transports N-methyl-D-aspartate (NMDA) receptor subunit NR2B along microtubules in neurons, mediated by the LIN-10-LIN-2-LIN-7 complex.
• In cilia, KIF17 collaborates with RAB23 and IFT57 to localize G protein-coupled receptors (e.g., dopamine receptor DRD1) to the primary cilium.
  Related references:
  PMID:26182404
• Subcellular localization encompasses the cytoplasm, cytoskeleton, cell projections (including dendrites and cilia), facilitating its functions in intracellular transport.
• Structural features include a coiled-coil domain for dimerization and an ATP-binding site, with alternative splicing generating multiple isoforms.
• Key associated biological processes include microtubule-based movement, protein transport, and ciliary trafficking.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 360–660) targets the stalk domain rather than the conserved motor domain, potentially reducing cross-reactivity with related kinesins.
• For Western blot, expect a band near 115 kDa; use lysates from brain tissue or neuronal cell lines (e.g., SH-SY5Y) known to express KIF17.
• In immunofluorescence-based assays (IF-P, IHC-P), optimize fixation/permeabilization to detect KIF17 at cilia, dendrites, and cytoplasm.
• The antibody recognizes human, mouse, and rat KIF17; validate species-specific reactivity in your experimental model.
• As a polyclonal antibody, lot-to-lot variability should be assessed, and titration is recommended for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-KIF17 polyclonal antibody supports research in neuronal transport and ciliary biology. Contact us for rare target sourcing or custom solutions—from concept to clinic.