Anti-KIF14 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15058

Core Product Specifications and Parameters

Biological Background: KIF14 Function and Localization

• KIF14 (Kinesin-like protein KIF14) is a microtubule motor protein with ATPase activity, belonging to the kinesin superfamily. Related references: PMID:16648480 PMID:24784001
• It plays critical roles in cell division, cytokinesis, cell proliferation, and apoptosis. Related references: PMID:16648480 PMID:24784001
• During cytokinesis, KIF14 targets the central spindle and midbody via interactions with PRC1 and CIT. Related references: PMID:16431929
• It regulates cell cycle progression by controlling CDKN1B degradation through SCF-dependent proteasomal ubiquitination, thus positively regulating cyclins CCNE1, CCND1, and CCNB1. Related references: PMID:24854087
• KIF14 is required for chromosome congression and alignment during mitosis. Related references: PMID:15843429
• It modulates cell spreading, focal adhesion dynamics, and migration by tethering RADIL on microtubules, affecting RAP1A-mediated integrin activation. Related references: PMID:23209302
• Subcellular localization includes nucleus, cytoplasm, spindle, and midbody.
• Keywords indicate involvement in ciliopathy, Meckel syndrome, primary microcephaly (disease variants), and it is a phosphoprotein.

Experimental Guidance and Technical Tips

• When performing Western blot, consider using a low-percentage gel (e.g., 6-8%) and extended transfer times due to the high molecular weight (186 kDa) of KIF14.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval and permeabilization steps may enhance signal; validate the antibody in known positive control tissues or cell lines.
• The immunogen corresponds to the C-terminal region (aa 1399-1648), which may provide isoform specificity; however, cross-reactivity with other kinesins should be assessed using appropriate controls.
• As a polyclonal antibody, lot-to-lot variability can occur; it is recommended to include positive and negative controls in each experiment and to titrate the antibody for the specific assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions by offering a comprehensive portfolio of IVD raw materials. From validated antibody pairs and monoclonal/polyclonal antibodies to bulk ancillary reagents, we facilitate seamless sourcing for targets like KIF14 involved in cell division and cytokinesis. Whether you need rare target reagents or established antibodies, CamelBio streamlines your development workflow from concept to clinic.