Anti-KIF13A Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H1H9

Core Product Specifications and Parameters

Biological Background: KIF13A Function and Localization

• KIF13A (kinesin-like protein KIF13A, also known as RBKIN) is a plus-end directed microtubule-dependent motor protein that converts chemical energy from ATP hydrolysis into mechanical force for intracellular transport.
• It plays key roles in mannose-6-phosphate receptor (M6PR) transport to the plasma membrane, endosomal sorting during melanosome biogenesis, and the abscission step of cytokinesis.
• KIF13A mediates transport of M6PR-containing vesicles from the trans-Golgi network to the plasma membrane via direct interaction with the AP-1 complex.
• During melanosome maturation, it delivers melanogenic enzymes from recycling endosomes to nascent melanosomes by creating peripheral recycling endosomal subdomains in melanocytes.
• In cytokinesis, KIF13A translocates ZFYVE26 (and possibly TTC19) to the midbody, which is essential for abscission.
• The protein localizes to the cytoplasm, cytoskeleton, centrosome, midbody, endosome membrane, and Golgi apparatus membrane.
• KIF13A is widely expressed, with highest levels in heart, brain, and skeletal muscle.
• It belongs to the kinesin motor protein family and features ATP-binding, coiled-coil, and microtubule-binding domains; post-translational phosphorylation has been reported.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1300–1600 in the C-terminal region, which may confer specific recognition of KIF13A; cross-reactivity with mouse is expected based on sequence homology.
• For Western blotting, the predicted molecular weight is ~202 kDa; use an appropriate gel percentage (e.g., 6–8% SDS-PAGE) and extended transfer conditions to ensure efficient blotting of high molecular weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols to visualize KIF13A at expected subcellular structures such as the centrosome, midbody, or endosomal membranes.
• For ELISA, consider using the antibody as a capture or detection reagent in a sandwich format; confirm suitability in your specific assay system.
• Always validate the antibody’s performance in the relevant sample system and include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by providing validated raw materials, including this anti-KIF13A polyclonal antibody, to support research in intracellular trafficking, melanosome biogenesis, and cell division. As a trusted partner, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets—bridging the gap from concept to clinic. Let us help you accelerate your diagnostic assay development with reliable, one-stop access to critical raw materials.