Anti-KIAA0513 Rabbit Polyclonal Antibody for WB, ELISA - O60268

Core Product Specifications and Parameters

Biological Background: KIAA0513 Function and Localization

• KIAA0513 is an uncharacterized protein encoded by the KIAA0513 gene in humans, with evidence of alternative splicing producing multiple isoforms.
• The protein is predicted to reside in the cytoplasm, as indicated by subcellular localization data, suggesting roles in cytoplasmic processes.
• KIAA0513 is widely expressed, with highest levels in specific brain regions including cerebellum, cortex, hippocampus, and amygdala, implying a potential role in neuronal function.
• Expression is detected in both neurons and glial cells, and notably, slightly elevated expression has been observed in the dorsolateral prefrontal cortex of schizophrenic patients, hinting at possible involvement in neuropsychiatric disorders.
• Post-translational modifications include phosphorylation, as the protein is classified as a phosphoprotein, which may regulate its activity or interactions.
• The protein is part of the reference proteome, indicating its canonical representation in human biology, though its exact molecular function remains to be elucidated.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning the 1-301 amino acid region of human KIAA0513, which corresponds to the protein's N-terminal portion; this design may provide specific detection in applications like WB.
• For Western blotting, consider optimizing antibody dilution and sample preparation conditions, especially given the predicted molecular weight of ~47 kDa and endogenous expression patterns.
• When using ELISA, ensure the recombinant antigen or target protein is properly immobilized, and validate the assay's linear range with known concentrations.
• Due to cross-reactivity with mouse and rat, this antibody can be a useful tool for comparative studies across these species; however, each application should be validated in the relevant sample system.
• Since KIAA0513 shows tissue-specific expression, include appropriate positive tissue controls (e.g., brain lysates) to confirm antibody specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research labs with a comprehensive range of IVD raw materials, including antibodies for elusive targets like KIAA0513. From validated antibody pairs to bulk ancillary reagents, our one-stop platform streamlines procurement for neuroscience and protein expression studies, connecting you from concept to clinic.