Anti-KIAA0196 (WASHC5) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q12768

Core Product Specifications and Parameters

Biological Background: WASHC5 (KIAA0196) Function and Localization

• As a core subunit of the WASH complex, WASHC5 acts as a nucleation-promoting factor at the endosome surface, recruiting Arp2/3 to initiate actin polymerization necessary for membrane tubule fission during cargo sorting (PubMed:19922875; PubMed:20498093). Related references: PMID:19922875 PMID:20498093
• Plays a role in axonal outgrowth, likely via its actin-regulatory activity.
• Regulates the cellular localization of the beta-2 adrenergic receptor (ADRB2) (PubMed:23085491). Related references: PMID:23085491
• Facilitates intracellular trafficking of BLOC-1-dependent cargos, including the copper transporter ATP7A and the SNARE protein VAMP7 (PubMed:23676666). Related references: PMID:23676666
• Predominantly localizes to the cytoplasm, including the cytosol, endoplasmic reticulum, and early endosomal compartments.
• Expressed ubiquitously across human tissues, consistent with its housekeeping roles in endosomal sorting.
• Mutations in WASHC5 are linked to autosomal dominant hereditary spastic paraplegia type 8 (SPG8) and autosomal recessive Ritscher-Schinzel syndrome (RTSC1), both involving neurodegeneration.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 380-530 of human KIAA0196; in Western blot, a band corresponding to the full-length protein at approximately 134 kDa may be observed—validate detection in your specific sample system.
• For immunohistochemistry (paraffin), consider the predominantly cytoplasmic localization of WASHC5 and optimize antigen retrieval conditions to enhance signal.
• This antibody cross-reacts with mouse and rat homologs, enabling multispecies comparative studies; confirm reactivity in your target tissue or cell line.
• In ELISA applications, pairing with a detection antibody against a non-overlapping epitope may improve assay sensitivity—titrate both antibodies to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions, including validated antibodies like this anti-WASHC5 polyclonal antibody, to support research and diagnostic development in endosomal trafficking and neurodegenerative disorders. Our portfolio encompasses optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw materials, ensuring streamlined procurement from concept to clinic. Contact us to explore tailored sourcing options for your next project.