Anti-Ki67 Rabbit PolymAb® (KO Validated) for WB, IF/ICC, IF-P, IHC-P, ELISA - E9PVX6

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• MKI67 (Ki-67) is a well-established proliferation marker expressed in the nucleus of actively dividing cells, with expression levels closely correlated with the cell cycle.
• It associates with mitotic chromosomes during early mitosis, forming a characteristic brush-like structure that acts as a steric and electrostatic barrier to prevent chromosome clustering. Related references: PMID:27362226
• The high net charge of Ki67 contributes to its surfactant-like properties, dispersing chromosomes and facilitating independent motility. Related references: PMID:27362226
• In anaphase, Ki67 switches to a chromosome attractant, promoting chromosome clustering through dephosphorylation and RNA-dependent liquid-liquid phase separation.
• It binds DNA, particularly supercoiled and AT-rich sequences, and interacts with premature ribosomal RNAs to modulate chromosomal organization.
• Ki67 localizes to the chromosome surface, nucleolus, and nucleus. Post-translational modifications include acetylation and phosphorylation.
• The protein has a predicted molecular weight of ~350 kDa, with alternative splicing leading to variants of 359 kDa.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from amino acids 2271–2370; consider including both positive and negative KO cell lysates for validation in WB.
• For WB, use a low-percentage gel (e.g., 6–8%) and extended transfer times due to the large protein size (~350 kDa).
• In IHC-P, nuclear Ki67 staining requires effective antigen retrieval; citrate buffer (pH 6.0) with heat is commonly used.
• For IF/ICC, a permeabilization step with 0.1–0.5% Triton X-100 is recommended to access nuclear antigens.
• This antibody is validated for knockout studies; verify specificity in your model system by comparing wild-type and KO samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, technical services, and consultancy to support diagnostic manufacturers and research laboratories from concept to clinic. This Ki67 PolymAb® targets a critical cell proliferation biomarker, making it ideal for immuno-oncology research, companion diagnostics, and prognostic assay development. As your one-stop sourcing partner, we provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring a reliable supply chain for your IVD development needs.