Anti-Ki67 Rabbit Polyclonal Antibody for IF/ICC, ELISA - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Proliferation marker protein Ki-67 (MKI67) associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and a chromosome attractant during late mitosis. Related references: PMID:27362226, PMID:32879492, PMID:35513709
• During early mitosis, Ki-67 relocalizes from nucleoli to the chromosome surface and forms extended brush-like structures that prevent chromosome collapse by providing a steric and electrostatic charge barrier. Related references: PMID:27362226
• At mitotic anaphase, dephosphorylation of Ki-67 and exposure of a conserved basic patch induce RNA-dependent liquid-liquid phase separation on the chromosome surface, promoting chromosome clustering and exclusion of large cytoplasmic particles from the future nuclear space. Related references: PMID:32879492, PMID:39153474, PMID:28935370
• Ki-67 binds to supercoiled DNA and AT-rich DNA, as well as premature ribosomal RNAs, which contributes to its role in mitotic chromosome organization and maintenance. Related references: PMID:10878551, PMID:24867636
• The protein localizes to nuclei in interphase cells, particularly concentrated in nucleoli, and associates with mitotic chromosomes during cell division (Subcellular location: Chromosome, Nucleus, Nucleolus).
• Ki-67 undergoes multiple post-translational modifications including phosphorylation, acetylation, and ubiquitination-like modifications (isopeptide bond, Ubl conjugation), with phosphorylation/dephosphorylation regulating its chromosome association and phase separation properties. Related references: PMID:39153474

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 700-800 of human Ki67; this region lies within the central repetitive domain and is likely to be accessible in the native protein.
• For IF/ICC, consider optimizing cell fixation and permeabilization to preserve nucleolar and mitotic chromosome staining. Proliferating cells such as HeLa or Jurkat can serve as positive controls.
• For ELISA, validate antibody reactivity using recombinant Ki67 fragments or whole-cell lysates from proliferating versus quiescent cells.
• Since the antibody recognizes human Ki67, cross-reactivity with other species is not expected unless there is high sequence homology in the immunogen region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. For Ki-67, a key proliferation marker in oncology and pathology, we offer this rabbit polyclonal antibody as part of a curated portfolio supporting cell cycle and cancer diagnostic development. Our sourcing capabilities also cover rare targets and custom immunogen design, ensuring a streamlined supply chain from concept to clinic.