Anti-Ki67 Polyclonal Antibody for WB, IHC-P, ELISA - P46013

Core Product Specifications and Parameters

Biological Background: Ki67 Function and Localization

• Ki67, encoded by the MKI67 gene, is a well-established proliferation marker protein (also known as Antigen KI-67 or Antigen Ki67).
• During early mitosis, Ki67 relocalizes from nucleoli to the chromosome surface, forming extended brush-like structures. These highly charged brushes act as a surfactant, preventing chromosome aggregation via steric and electrostatic repulsion. Related references: PMID:27362226
• In anaphase, Ki67 switches to a chromosome attractant, undergoing dephosphorylation and RNA-dependent liquid-liquid phase separation to promote chromosome clustering. Related references: PMID:32879492, PMID:39153474, PMID:28935370
• Ki67 binds DNA with a preference for supercoiled and AT-rich sequences, and also interacts with premature ribosomal RNAs during anaphase. Related references: PMID:10878551, PMID:28935370
• It may also contribute to chromatin organization, though the direct mechanism remains unclear. Related references: PMID:24867636
• Ki67 localizes to the nucleolus during interphase and to the chromosome surface during mitosis.
• The protein is subject to various post-translational modifications including phosphorylation, acetylation, isopeptide bond formation, and ubiquitin conjugation.

Experimental Guidance and Technical Tips

• The recombinant immunogen covers amino acids 460-680 of human Ki67 (NP_002408.3), which may influence epitope specificity. Consider this when designing detection assays.
• For western blot, note the high molecular weight (~359 kDa); use low-percentage SDS-PAGE gels and extended transfer times or wet transfer methods to ensure efficient transfer.
• For IHC-P, validate antigen retrieval conditions and primary antibody dilution in the relevant sample system. Include appropriate positive (e.g., proliferating tissues) and negative controls.
• This antibody shows cross-reactivity with mouse, enabling its use in xenograft or murine model studies; however, verify species-specific performance experimentally.
• ELISA applications require optimization of coating and detection protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers from concept to clinic. Our Ki67 polyclonal antibody addresses critical needs in cell proliferation and oncology biomarker research. In addition to this product, we supply validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and rare target raw materials to streamline your assay development workflows.