Anti-KHDRBS3 Polyclonal Antibody for WB, IF/ICC, ELISA - O75525

Core Product Specifications and Parameters

Biological Background: KHDRBS3 Function and Localization

• KHDRBS3 (KH domain-containing, RNA-binding, signal transduction-associated protein 3) is a member of the STAR family of RNA-binding proteins that links signal transduction to post-transcriptional regulation.
• It binds specifically to the 5'-[AU]UAAA-3' RNA motif and poly(A), with RNA-binding activity down-regulated by tyrosine phosphorylation through PTK6. Related references: PMID:10564820 PMID:19561594 PMID:26758068
• KHDRBS3 is involved in splice site selection of vascular endothelial growth factor (VEGF) pre-mRNA, influencing angiogenic signaling. Related references: PMID:15901763
• It regulates alternative splicing of CD44 and neurexins (NRXN1-3) at the AS4 site, modulating cell adhesion and synaptic targeting.
• Localized predominantly to the nucleus, consistent with its role in splicing regulation.
• Ubiquitously expressed, with higher levels in testis, skeletal muscle, brain, and kidney podocytes; expression is enhanced after meiosis.
• KHDRBS3 also plays a role in post-transcriptional regulation of HIV-1 gene expression, contributing to viral replication.
• It may act as a negative regulator of cell growth by inhibiting proliferation.

Experimental Guidance and Technical Tips

• This antibody was raised against amino acids 177-346 of human KHDRBS3; the predicted molecular weight is 39 kDa, though actual migration may vary due to post-translational modifications.
• For Western blotting, consider using whole-cell lysates from human or mouse tissues/cells with appropriate controls.
• In IF/ICC, a predominantly nuclear signal is expected; validate the staining pattern in a relevant cell line.
• For ELISA, the antibody can be used for detection of recombinant or endogenous KHDRBS3; optimization of coating and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research groups with reliable access to IVD raw materials, including validated polyclonal antibodies like this anti-KHDRBS3 antibody for alternative splicing studies. We offer one-stop procurement covering antibody pairs, bulk ancillary reagents, and rare-target antibody sourcing to accelerate your assay development from concept to clinic.