Anti-KHDRBS1/Sam68 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q07666

Core Product Specifications and Parameters

Biological Background: KHDRBS1/Sam68 Function and Localization

• KHDRBS1/Sam68, also known as p62, p68, or GAP-associated tyrosine phosphoprotein p62, is an RNA-binding protein that belongs to the KH domain-containing family. It exists in multiple isoforms, with isoform 1 being the canonical form and isoform 3 induced in growth-arrested cells.
• Acts as a key adapter protein in signal transduction cascades: recruited and tyrosine-phosphorylated by receptors such as T-cell, leptin, and insulin receptors, then binds SH2 and SH3 domain-containing proteins to propagate signals.
• Plays a role in G2-M cell cycle progression and has isoform-specific functions; isoform 3 inhibits S phase when expressed in growth-arrested cells.
• Regulates alternative splicing of multiple transcripts: promotes exon inclusion or skipping in targets like CD44 (Ras pathway-dependent), BCL2L1 (favors Bcl-X(S) isoform), SMN1, NRXN1, and NRXN3. Related references: PMID:26080397 PMID:17371836 PMID:20186123
• Involved in mRNA metabolism, including regulation of stability, translation, and nuclear export. Positively regulates translation of CTE-containing mRNAs, though some studies dispute its role in nucleocytoplasmic export of unspliced CTE-RNAs. Related references: PMID:22253824
• Subcellular localization is dynamic: found in the nucleus, cytoplasm, and at the membrane, consistent with its roles in signaling and RNA processing.
• Ubiquitously expressed across tissues, with isoform 1 levels lower in brain, skeletal muscle, and liver, while isoform 3 is intensified in skeletal muscle and liver.
• Subject to multiple post-translational modifications including tyrosine phosphorylation, acetylation, methylation, and ubiquitination, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 343-443 of human KHDRBS1/Sam68, targeting a region that may be conserved across species, supporting reactivity with human, mouse, and rat.
• For Western blotting, consider the predicted molecular weight of ~48 kDa for the canonical isoform; additional bands may represent isoforms or modified forms.
• In IHC-P and IF/ICC, nuclear and cytoplasmic staining is expected; membrane localization may also be observed depending on cell type and signaling state. Validate staining patterns with appropriate controls.
• The antibody is suitable for ELISA, but optimal working dilutions should be determined empirically for each assay format.
• As a polyclonal antibody, it may detect multiple isoforms and potentially cross-react with closely related KHDRBS family members; confirm specificity in knockout or knockdown models if precise isoform discrimination is required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. For studies on signal transduction, RNA biology, or cell cycle regulation, this KHDRBS1/Sam68 antibody serves as a reliable tool, and CamelBio can further assist in sourcing matched antibody pairs, bulk quantities, or custom formulations. Whether you need individual components or integrated workflows, CamelBio provides end-to-end solutions from concept to clinic.