Anti-KEAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q14145

Core Product Specifications and Parameters

Biological Background: KEAP1 Function and Localization

• KEAP1 (Kelch-like ECH-associated protein 1), also referred to as INrf2 or KLHL19, is a 70 kDa cytoplasmic and nuclear protein that functions as a substrate-specific adapter for the BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex.
• Under basal conditions, KEAP1 targets the transcription factor NFE2L2/NRF2 for ubiquitination and proteasomal degradation, thereby repressing the expression of cytoprotective genes. Related references: PMID:14585973 PMID:15379550 PMID:15572695 PMID:15601839 PMID:16006525
• As a key oxidative stress sensor, KEAP1 contains highly reactive cysteine residues that undergo non-enzymatic covalent modification by electrophiles, leading to inactivation of the ligase complex and subsequent nuclear accumulation of NRF2. Related references: PMID:16006525 PMID:17127771 PMID:18251510
• KEAP1 also participates in selective autophagy by interacting with SQSTM1/p62; this sequesters KEAP1 into inclusion bodies, further activating NRF2, while simultaneously mediating ubiquitination and degradation of SQSTM1/p62. Related references: PMID:20452972 PMID:28380357
• Additional substrates of the KEAP1-containing ubiquitin ligase complex include BPTF and PGAM5, highlighting its broad regulatory roles. Related references: PMID:15379550 PMID:17046835
• KEAP1 is broadly expressed in human tissues, with the highest levels found in skeletal muscle; the protein contains characteristic Kelch repeats and a BTB domain, and is subject to post-translational modifications including S-nitrosylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 320–510 of human KEAP1; this region may allow recognition of full-length KEAP1 and potentially conserved epitopes across species. Verify specificity in your sample system.
• For Western blotting, expect a band corresponding to the predicted molecular weight of approximately 70 kDa. Include appropriate positive and negative controls.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), KEAP1 can be detected in both cytoplasmic and nuclear compartments. Consider optimizing fixation and permeabilization steps.
• The reported cross-reactivity includes human, mouse, and rat; however, performance in each species should be validated with relevant tissues or cell lines.
• For ELISA, the recombinant protein immunogen suggests potential use in capture or detection setups; confirm binding affinity and specificity with target and control proteins.

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