Anti-KDM7A Rabbit Polyclonal Antibody for WB, ELISA - Q3UWM4

Core Product Specifications and Parameters

Biological Background: Lysine-specific demethylase 7A (KDM7A) Function and Localization

• Lysine-specific demethylase 7A (KDM7A), also known as JmjC domain-containing histone demethylation protein 1D (Jhdm1d), is a histone demethylase belonging to the JmjC domain-containing protein family.
• It specifically catalyzes the demethylation of dimethylated histone H3 at lysine 9, 27, and 36 (H3K9me2, H3K27me2, H3K36me2) and monomethylated histone H4 at lysine 20 (H4K20me1), functioning as an epigenetic eraser.
• Demethylase activity is modulated by binding to trimethylated H3K4 (H3K4me3); in the presence of H3K4me3, KDM7A preferentially demethylates H3K27me2 over H3K9me2, and it requires a nucleosome substrate for H4K20me1 demethylation.
• KDM7A is essential for brain development and neurogenesis, highlighting its role in neuronal chromatin regulation.
• The protein localizes to the nucleus, consistent with its involvement in chromatin remodeling and transcriptional control.
• KDM7A contains a JmjC domain requiring iron and zinc as cofactors, and is regulated by phosphorylation. It acts as an oxidoreductase, modulating transcriptional programs.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant fragment corresponding to amino acids 100–200 of mouse KDM7A. It may recognize both full-length and truncated isoforms; cross-reactivity with other JmjC family members should be experimentally validated.
• Recommended for Western blot (WB) under denaturing and reducing conditions. Use nuclear extracts to enrich for KDM7A, and ensure proper separation of the ~106 kDa protein on SDS-PAGE (e.g., 8–10% gel, extended transfer time for high molecular weight).
• For ELISA, consider using the antibody as a capture or detection reagent after confirming binding to the immunogen or purified antigen in a standard indirect format.
• As with any polyclonal antibody, lot-to-lot consistency may vary; we recommend titrating the antibody and including appropriate positive (e.g., mouse brain lysate) and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials. This anti-KDM7A rabbit polyclonal antibody exemplifies our commitment to delivering validated tools for epigenetics and neuroscience research. Whether you require optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, or sourcing for challenging targets, CamelBio is your dedicated partner from concept to clinic.