Anti-KDM7A Rabbit Polyclonal Antibody for WB and ELISA - Q3UWM4

Core Product Specifications and Parameters

Biological Background: KDM7A/Lysine-specific demethylase 7A Function and Localization

• KDM7A, also known as lysine-specific demethylase 7A or JHDM1D, is a histone demethylase required for brain development.
• It specifically demethylates dimethylated lysine-9, lysine-27, and lysine-36 on histone H3 (H3K9me2, H3K27me2, H3K36me2) and monomethylated lysine-20 on histone H4 (H4K20me1), playing a central role in the histone code.
• The demethylase activity of KDM7A is regulated by H3K4me3 binding: in the presence of H3K4me3, it demethylates H3K27me2 but not H3K9me2; in the absence of H3K4me3, it demethylates H3K9me2.
• KDM7A preferentially demethylates H4K20me1 in the context of nucleosomes rather than histone octamers.
• Subcellular localization is nuclear.
• KDM7A belongs to the JmjC domain-containing family and functions as an iron-dependent dioxygenase with a zinc-finger domain; it is a phosphoprotein involved in transcription regulation.

Experimental Guidance and Technical Tips

• For Western blot (WB), consider using a dilution range of 1:500 to 1:2000 and validate in your specific cell or tissue lysates. The predicted molecular weight is approximately 106 kDa, but alternative splicing may yield isoforms around 92 kDa.
• For ELISA, titrate the antibody to determine the optimal working concentration. Pair it with a suitable detection system.
• As a polyclonal antibody, it may recognize multiple isoforms and post-translationally modified forms; this can be advantageous for detecting total KDM7A levels.
• Validate the antibody’s reactivity in the relevant sample systems (human, mouse, or rat) to ensure specificity for your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-KDM7A polyclonal antibody is ideal for studies in epigenetics and brain development, and we can supply it alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to streamline your assay development.