Anti-KDM6A (UTX) Rabbit mAb [KD Validated] for WB, IF-P, IHC-P, ELISA - O15550

Core Product Specifications and Parameters

Biological Background: KDM6A Function and Localization

• KDM6A, also known as lysine-specific demethylase 6A or UTX, is a histone demethylase that specifically removes methyl groups from trimethylated and dimethylated lysine 27 of histone H3 (H3K27me3/me2), playing a crucial role in histone code regulation. Related references: PMID:17713478, PMID:17761849, PMID:17851529
• Demethylation of H3K27 by KDM6A is coupled with methylation of H3K4 and regulates recruitment of the PRC1 complex and monoubiquitination of H2A, highlighting its role in Polycomb group protein regulation. Related references: PMID:17761849
• KDM6A is essential for posterior development by controlling HOX gene expression, demonstrating its importance in developmental processes. Related references: PMID:17851529
• In addition to its demethylase activity, KDM6A has a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression (By similarity).
• KDM6A localizes predominantly to the nucleus, consistent with its function in epigenetic chromatin modification.
• Post-translational modifications of KDM6A include phosphorylation, and it contains TPR repeat domains, which are implicated in protein-protein interactions.
• Mutations in KDM6A are associated with Kabuki syndrome 2 (KABUK2) and intellectual disability, highlighting its clinical significance.

Experimental Guidance and Technical Tips

• Immunogen design: The synthetic peptide immunogen spanning amino acids 611-710 of human KDM6A (NP_066963.2) is likely to generate antibodies recognizing both endogenous and recombinant KDM6A. Consider verifying sequence homology across target species to ensure cross-reactivity.
• Western blot (WB): A major band corresponding to the predicted molecular weight of approximately 154 kDa is expected. Validate antibody specificity in the relevant sample system, and optimize loading controls.
• Immunofluorescence (IF-P) and immunohistochemistry (IHC-P): Since KDM6A is a nuclear protein, ensure adequate permeabilization and nuclear counterstaining for clear localization. The antibody's reactivity with human, mouse, and rat samples allows for broad model organism validation.
• ELISA: The antibody can be used as a capture or detection reagent in ELISA formats; determine optimal coating concentrations and detection ranges experimentally.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research teams with reliable access to high-performance IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. This anti-KDM6A antibody serves as a robust tool for epigenetic research, particularly in chromatin biology and developmental studies. Let our expert team streamline your procurement process from early-stage discovery to clinical application.