Anti-KDM5B Rabbit Polyclonal Antibody [KO Validated] for WB, IF/ICC, ELISA - Q9UGL1

Core Product Specifications and Parameters

Biological Background: KDM5B Function and Localization

• Histone demethylase that specifically demethylates 'Lys-4' of histone H3 (H3K4me3, H3K4me2, H3K4me1), playing a central role in histone code regulation. Related references: PMID:24952722 PMID:27214403 PMID:28262558
• Does not demethylate histone H3 'Lys-9' or 'Lys-27', showing high substrate specificity.
• Acts as a transcriptional corepressor for FOXG1B and PAX9, linking chromatin modification to developmental gene regulation.
• Favors breast cancer cell proliferation by repressing tumor suppressor genes such as BRCA1 and HOXA5. Related references: PMID:24952722
• May function as a tumor suppressor in melanoma, indicating context-dependent roles in cancer.
• Represses the CLOCK-BMAL1 heterodimer-mediated transcriptional activation of the core clock component PER2, suggesting a role in circadian rhythm regulation.
• Localized in the nucleus, consistent with its function as a chromatin-modifying enzyme.
• Ubiquitously expressed, with highest levels in testis; downregulated in melanoma and glioblastoma; upregulated in breast cancer.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a recombinant fusion protein covering amino acids 784-883 of human KDM5B, a region located within the JmjC domain critical for demethylase activity. Consider verifying antibody specificity in knockout models given the KO validation.
• For Western blot, a band at approximately 176 kDa is expected; use appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In IF/ICC, confirm nuclear localization of KDM5B; include appropriate controls to account for any non-specific staining.
• For ELISA, validate antibody performance in the assay of choice, considering standard optimization protocols.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-KDM5B polyclonal antibody supports epigenetic and cancer research applications. Beyond this product, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, streamlining your path from concept to clinic.