Anti-KDM5A Polyclonal Antibody for WB, IP, ELISA, ChIP - P29375

Core Product Specifications and Parameters

Biological Background: KDM5A Function and Localization

• Lysine-specific demethylase 5A (KDM5A), also known as JARID1A, RBBP-2, or RBP2, is a histone demethylase that specifically removes methyl groups from trimethylated and dimethylated lysine 4 of histone H3 (H3K4), playing a pivotal role in chromatin regulation and gene transcription.
• KDM5A specifically demethylates H3K4me3 and H3K4me2 but not monomethylated H3K4, nor other histone lysine residues (H3K9, H3K27, H3K36, H3K79, H4K20), thereby contributing to histone code dynamics.
• It regulates gene transcription by binding directly to DNA at the 5'-CCGCCC-3' motif, impacting downstream target gene expression. Related references: PMID:18270511
• KDM5A is involved in transcriptional control of Hox proteins during cell differentiation, and may repress cytokines like CXCL12, as well as regulate circadian rhythm through a demethylase-independent coactivator function with CLOCK-BMAL1. Related references: PMID:19430464
• The protein localizes to the nucleus and nucleolus, consistent with its role in chromatin modification and transcription regulation.
• KDM5A has been reported to act as a transcriptional corepressor for certain genes (e.g., MT1F) and to favor the proliferation of cancer cells, highlighting its potential significance in oncology. Related references: PMID:27427228
• As a Jumonji/ARID domain-containing protein, KDM5A belongs to the JARID family of histone demethylases, which are characterized by their oxygenase activity requiring iron and 2-oxoglutarate.

Experimental Guidance and Technical Tips

• For Western blot (WB), given the large molecular weight of KDM5A (~192 kDa), use low-percentage acrylamide gels and ensure thorough protein transfer with appropriate buffers and extended transfer times.
• In immunoprecipitation (IP), the polyclonal nature of this antibody may enhance capture efficiency, but include isotype controls and validate recovery by target-specific detection.
• For chromatin immunoprecipitation (ChIP), this antibody is predicted to work based on the recombinant full-length immunogen; however, it is advisable to titrate the antibody and confirm enrichment of known KDM5A target promoters in your experimental system.
• ELISA applications may require optimization of coating antigen and antibody dilution; consider using the recombinant immunogen as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutions with integrated access to high-quality IVD raw materials, including validated antibody pairs, recombinant proteins, and bulk reagents. For KDM5A-related research and assay development, we offer polyclonal antibodies suited for WB, IP, ELISA, and ChIP, alongside flexible sourcing for ancillary reagents. Our commitment is to streamline your supply chain, helping you move from concept to clinic with reliable and efficient raw-material procurement.