Anti-KDM4C Rabbit Polyclonal Antibody for WB, ELISA - Q9H3R0

Core Product Specifications and Parameters

Biological Background: KDM4C Function and Localization

• KDM4C, also known as JMJD2C or GASC1, is a member of the Jumonji C (JmjC) domain-containing histone demethylase family that specifically demethylates trimethylated histone H3 lysine 9 (H3K9me3) and lysine 36 (H3K36me3), but has no activity on mono- or dimethylated residues.
• It does not demethylate histone H3 lysine 4 (H3K4) or lysine 27 (H3K27), nor histone H4 lysine 20 (H4K20), demonstrating its substrate specificity.
• Demethylation by KDM4C produces formaldehyde and succinate as byproducts, requiring iron and α-ketoglutarate as cofactors.
• The protein contains JmjN and JmjC domains, as well as zinc-finger motifs, which contribute to its chromatin targeting and catalytic activity.
• KDM4C is primarily localized in the nucleus, consistent with its role in transcriptional regulation and chromatin remodeling.
• Overexpression of KDM4C has been observed in several esophageal squamous cell carcinomas (ESCs), implicating it in cancer development.
• Alternative splicing of the KDM4C gene generates multiple isoforms, though their functional differences are not fully characterized.

Experimental Guidance and Technical Tips

• For Western blot analysis, detecting a band at approximately 120 kDa may require optimized reducing conditions and extended transfer times due to the high molecular weight.
• When using this polyclonal antibody in ELISA, it can be employed as either a capturing or detecting antibody, though pairing should be validated with the specific assay setup.
• Given the high sequence homology of KDM4C between human, mouse, and rat, this antibody is expected to cross-react; however, it is recommended to verify reactivity in your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes streamlined access to high-quality antibodies and raw materials for IVD development. This KDM4C polyclonal antibody supports epigenetic and oncology research applications, with CamelBio providing sourcing for validated antibody pairs, monoclonal antibodies, and bulk ancillary reagents to accelerate your project from concept to clinic.