Anti-KDM4B/JMJD2B Rabbit Polyclonal Antibody for WB - O94953

Core Product Specifications and Parameters

Biological Background: KDM4B/JMJD2B Function and Localization

• KDM4B, also known as JMJD2B or JHDM3B, is a lysine-specific demethylase that targets histone H3 trimethylated at lysine 9 (H3K9me3), converting it to dimethylated and monomethylated forms, thereby regulating chromatin structure and gene expression. Related references: PMID:16603238 PMID:28262558
• The enzyme belongs to the JmjC domain-containing histone demethylase family and contains several conserved domains, including JmjN, JmjC, and two PHD-type zinc fingers, which are critical for its catalytic activity and chromatin binding.
• KDM4B does not demethylate H3K4, H3K27, H3K36, or H4K20, demonstrating high substrate specificity for the trimethylated H3K9 mark.
• It is predominantly localized in the nucleus, consistent with its role in epigenetic regulation and chromatin remodeling.
• Alternative splicing produces multiple isoforms, and the protein is subject to post-translational modifications such as acetylation and phosphorylation, which modulate its activity and stability.
• KDM4B plays a critical role in the development of the central nervous system (CNS), and mutations in the gene are associated with intellectual disability, highlighting its importance in neurodevelopment.
• The protein's demethylation reaction requires iron and alpha-ketoglutarate as cofactors, classifying it as a Fe(II)- and 2-oxoglutarate-dependent dioxygenase.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant protein covering the N-terminal region (aa 1-365), which may generate antibodies recognizing multiple isoforms or conserved domains; consider sequence alignment to predict cross-reactivity.
• For Western blotting, a band at approximately 122 kDa is expected; validate in the relevant sample system and optimize antibody dilution as needed.
• ELISA applications may be useful for screening and titration; however, specific protocols should be established independently.
• Cross-reactivity with human and mouse is reported; for other species, check sequence homology before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, technical services, and consulting, providing a seamless bridge from concept to clinic. This anti-KDM4B antibody supports research into epigenetic regulation and neurological disorders, aligning with CamelBio’s capability to supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing for diagnostic manufacturers and laboratories.