Anti-KDM4A Rabbit Monoclonal Antibody for WB, IP, ELISA - O75164

Core Product Specifications and Parameters

Biological Background: KDM4A Function and Localization

• Lysine-specific demethylase 4A (KDM4A), also known as JMJD2A or JHDM3A, is a histone demethylase that specifically demethylates trimethylated 'Lys-9' (H3K9me3) and 'Lys-36' (H3K36me3) of histone H3, with no activity on mono- or dimethylated residues or on H3K4, H3K27, or H4K20. It also demethylates histone H1.4 at 'Lys-26' (H1.4K26me1/me2/me3).
  Related references: PMID:16603238, PMID:26741168, PMID:21768309, PMID:19144645, PMID:30156264
• KDM4A participates in transcriptional repression by recruiting histone deacetylases (HDACs) and the co-repressor NCOR1 to E2F-responsive and ASCL2 promoters.
  Related references: PMID:16024779
• A muscle-specific isoform of KDM4A lacking the N-terminal demethylase domain is crucial for muscle differentiation; it activates Myog gene transcription by removing repressive chromatin marks.
• KDM4A is localized to the nucleus.
• KDM4A is ubiquitously expressed across human tissues.
• The protein belongs to the JmjC domain-containing histone demethylation family and contains zinc-finger domains. It is subject to acetylation, phosphorylation, and ubiquitination, and its activity involves iron and zinc as cofactors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 382-459 of human KDM4A, located in the N-terminal region. Verify that the antibody detects both full-length and muscle-specific isoforms lacking the N-terminal domain by testing in relevant cell lysates (e.g., myoblasts vs. myotubes).
• For Western blot, a band at ~121 kDa is expected for full-length KDM4A; validate using nuclear extracts from human, mouse, or rat cell lines.
• Optimize antibody dilution for each application (WB, IP, ELISA) using positive control samples; perform titration experiments to determine optimal working concentration.
• For immunoprecipitation, consider cross-linking methods or direct protocols based on antibody isotype and target abundance.
• Include appropriate controls (e.g., isotype control, knockout lysates) to confirm specificity.

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CamelBio is your integrated partner for IVD raw material sourcing, offering a comprehensive portfolio of antibodies, antigen pairs, and ancillary reagents to diagnostic developers and researchers worldwide. For epigenetic targets like KDM4A, we provide specialized supply solutions to accelerate your assay development from concept to clinic. Contact our team for bulk ordering, technical consultation, and custom request fulfillment.