Anti-KDM3B Polyclonal Antibody for WB, IHC-P, ELISA - Q7LBC6

Core Product Specifications and Parameters

Biological Background: KDM3B Function and Localization

• KDM3B is a lysine-specific demethylase that targets histone H3 dimethyl-Lys-9 (H3K9me2), converting it to mono- and unmethylated forms, thereby modulating chromatin structure and gene expression.
• The demethylation reaction requires iron and 2-oxoglutarate, producing formaldehyde and succinate as byproducts; this classifies KDM3B as a member of the JmjC domain-containing dioxygenase family.
• Encoded by the KDM3B gene (alias JMJD1B, JHDM2B), alternative splicing generates multiple isoforms; the full-length protein has a predicted molecular weight of ~192 kDa.
• KDM3B is primarily localized in the nucleus, consistent with its role in epigenetic regulation and transcription.
• Expression is ubiquitous, with highest levels in placenta, skeletal muscle, kidney, heart, and liver, suggesting tissue-specific regulatory functions.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination, potentially influencing its stability, activity, or subnuclear localization.
• KDM3B may possess tumor suppressor activity, and mutations in this gene have been associated with diseases such as dwarfism and intellectual disability (DIJOS, NET22).
• The protein contains a zinc-finger domain, which may mediate DNA or protein interactions critical for its chromatin-targeting function.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 400-600 of human KDM3B, which falls in a region outside the known catalytic JmjC domain; this may help detect total KDM3B levels or specific isoforms.
• For Western blot (WB), the reported protein weight is 192 kDa; ensure appropriate gel percentage and transfer conditions for high-molecular-weight proteins.
• In immunohistochemistry-paraffin (IHC-P), antigen retrieval methods should be optimized; consider testing sodium citrate or EDTA-based protocols.
• ELISA applications may require optimization of coating antigens and antibody titers; the antibody can be used as a capture or detection reagent in sandwich formats.
• Since cross-reactivity is listed only for human, validate reactivity in other species if needed; the antibody was raised against human antigen, so cross-reactivity with mouse or rat is not guaranteed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement pathway for high-quality IVD raw materials, including this anti-KDM3B polyclonal antibody for epigenetic research and diagnostic development. We supply validated antibody pairs, bulk ancillary reagents, and can assist in sourcing rare target-specific reagents to accelerate your assay development from concept to clinic.