Anti-KDM3A Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9Y4C1

Core Product Specifications and Parameters

Biological Background: KDM3A Function and Localization

• Encoded by the KDM3A gene (also known as JMJD1A, JHDM2A, TSGA), Lysine-specific demethylase 3A is a member of the JmjC domain-containing histone demethylase family.
• Functions as a dioxygenase that specifically demethylates mono- and dimethylated histone H3 at Lys-9 (H3K9me1/me2), with a preference for the dimethylated state, while exhibiting weak or no activity toward trimethylated H3K9.
• Plays a critical role in hormone-dependent transcriptional activation by being recruited to androgen receptor target genes, leading to local H3K9 demethylation and gene activation.
• Participates in spermatogenesis by regulating the expression of essential sperm chromatin packaging genes such as PRM1 and TNP1.
• Involved in metabolic regulation and obesity resistance through modulation of metabolic genes including PPARA and UCP1.
• Localizes to both the nucleus and cytoplasm, consistent with its roles in chromatin remodeling and potential cytoplasmic functions.
• Contains a JmjC domain and a zinc-finger domain, binding iron and zinc as cofactors; the protein undergoes acetylation and phosphorylation, as indicated by post-translational modification keywords.

Experimental Guidance and Technical Tips

• The immunogen peptide (aa 975-1283) lies within the C-terminal region of KDM3A; verify epitope accessibility in your specific assay format.
• For Western blot, a band around 147 kDa is expected; consider using a high-molecular-weight protein marker and optimized transfer conditions for large proteins.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), compare nuclear and cytoplasmic staining patterns; appropriate antigen retrieval and permeabilization may be necessary.
• This antibody is polyclonal; lot-to-lot consistency should be tested, and pre-adsorption controls may be used if non-specific bands appear.
• Validate antibody performance in your target tissue or cell line, especially for applications in testis or metabolic tissues where KDM3A is functionally relevant.

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CamelBio offers comprehensive sourcing solutions for IVD raw materials, including high-quality antibodies like this KDM3A polyclonal antibody for chromatin and transcription research. We provide validated antibody pairs, optimized monoclonals and polyclonals, bulk ancillary reagents, and customized rare-target sourcing to streamline your assay development from concept to clinic. Our one-stop platform supports diagnostic manufacturers, laboratories, and research institutions with technical consultation and reliable supply chain management.